Subbiah, Ramesh Jeon, Seong Beom Park, Kwideok Ahn, Sang Jung Yun, Kyusik 2024-01-20T06:32:10Z 2024-01-20T06:32:10Z 2021-09-05 2015-08 1176-9114 https://pubs.kist.re.kr/handle/201004/125168 In order for nanoparticles (NPs) to be applied in the biomedical field, a thorough investigation of their interactions with biological systems is required. Although this is a growing area of research, there is a paucity of comprehensive data in cell-based studies. To address this, we analyzed the physicomechanical responses of human alveolar epithelial cells (A549), mouse fibroblasts (NIH3T3), and human bone marrow stromal cells (HS-5), following their interaction with silver nanoparticles (AgNPs). When compared with kanamycin, AgNPs exhibited moderate antibacterial activity. Cell viability ranged from <80% at a high AgNPs dose (40 mu g/mL) to.95% at a low dose (10 mu g/mL). We also used atomic force microscopy-coupled force spectroscopy to evaluate the biophysical and biomechanical properties of cells. This revealed that AgNPs treatment increased the surface roughness (P < 0.001) and stiffness (P<0.001) of cells. Certain cellular changes are likely due to interaction of the AgNPs with the cell surface. The degree to which cellular morphology was altered directly proportional to the level of AgNP-induced cytotoxicity. Together, these data suggest that atomic force microscopy can be used as a potential tool to develop a biomechanics-based biomarker for the evaluation of NP-dependent cytotoxicity and cytopathology. English DOVE MEDICAL PRESS LTD ATOMIC-FORCE MICROSCOPY DIFFERENTIATION CELLS Investigation of cellular responses upon interaction with silver nanoparticles Article 10.2147/IJN.S88508 1 INTERNATIONAL JOURNAL OF NANOMEDICINE, v.10, pp.191 - 201 INTERNATIONAL JOURNAL OF NANOMEDICINE 10 191 201 scie scopus 000365122300020 2-s2.0-84947443063 Nanoscience & Nanotechnology Pharmacology & Pharmacy Science & Technology - Other Topics Pharmacology & Pharmacy Article ATOMIC-FORCE MICROSCOPY DIFFERENTIATION CELLS AFM roughness nanoindentation biomarker cytotoxicity biomechanics