Oh, Young Hoon Choi, Jae Woo Kim, Eun Young Song, Bong Keun Jeong, Ki Jun Park, Kyungmoon Kim, Il-Kwon Woo, Han Min Lee, Seung Hwan Park, Si Jae 2024-01-20T06:32:12Z 2024-01-20T06:32:12Z 2021-09-05 2015-08 0273-2289 https://pubs.kist.re.kr/handle/201004/125170 Corynebacterium glutamicum is an important microorganism in the biochemical industry for the production of various platform chemicals. However, despite its importance, a limited number of studies have been conducted on how to constitute gene expression cassettes in engineered C. glutamicum to obtain desired amounts of the target products. Therefore, in this study, six expression cassettes for the expression of the second lysine decarboxylase of Escherichia coli, LdcC, were constructed using six synthetic promoters with different strengths and were examined in C. glutamicum for the production of cadaverine. Among six expression cassettes, the expression of the E. coli ldcC gene under the PH30 promoter supported the highest production of cadaverine in flask and fed-batch cultivations. A fed-batch fermentation of recombinant C. glutamicum expressing E. coli ldcC gene under the PH30 promoter resulted in the production of 40.91 g/L of cadaverine in 64 h. This report is expected to contribute toward developing engineered C. glutamicum strains to have desired features. English SPRINGER RENEWABLE RESOURCES ESCHERICHIA-COLI ACID 5-AMINOVALERATE PATHWAY NYLON Construction of Synthetic Promoter-Based Expression Cassettes for the Production of Cadaverine in Recombinant Corynebacterium glutamicum Article 10.1007/s12010-015-1701-4 1 APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.176, no.7, pp.2065 - 2075 APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 176 7 2065 2075 scie scopus 000359432500020 2-s2.0-84939261206 Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Article RENEWABLE RESOURCES ESCHERICHIA-COLI ACID 5-AMINOVALERATE PATHWAY NYLON Cadaverine L-lysine Fermentation C. glutamicum Synthetic promoters