Akter, Hafeza Park, Min Kwon, Oh-Seung Song, Eun Joo Park, Won-Sang Kang, Min-Jung 2024-01-20T06:32:42Z 2024-01-20T06:32:42Z 2021-09-04 2015-08 1010-4283 https://pubs.kist.re.kr/handle/201004/125198 Neurotensin (NT) is distributed throughout the brain and gastrointestinal tract. Although the relationship between NT and matrix metalloproteinase-9 (MMP-9) activity in gastric cancer has not been reported, the elevation of MMP-9 and NT is reported in the breast, lung, prostate, and gastric cancer. The aim of our study is to investigate the relationship between NT and MMP-9 activity and the underlying signaling mechanism in gastric cancer cell lines. Commercial ELISA kits were used for estimation of NT and MMP-9 expression, and fluorescence resonance energy transfer (FRET) assay was used for measurement of MMP-9 activity. Cell migration and invasion were determined by wound healing and transwell assay. The expression of signaling proteins was measured by Western blotting. Our study reveals a positive correlation between increased plasma NT and MMP-9 activity in both of patient's serum and gastric cancer cell lines. A dose-dependent elevation of MMP-9 activity was observed by NT treatment in gastric cancer cells (MKN-1 and MKN-45) compared to untreated gastric cancer and normal epithelial cell (HFE-145). Moreover, NT-mediated migration and invasion were observed in gastric cancer cells unlike in normal cell. The signaling mechanism of NT in gastric cancer cells was confirmed in protein kinase C (PKC), extracellular-signal regulated kinase (ERK), and phosphatidylinositol 3-kinase (PI3K) pathway. In addition, pretreatment of gastric cancer cells with NTR1 inhibitor SR48692 was shown to significantly inhibit the NT-mediated MMP-9 activity, cell invasion, and migration. Our finding illustrated NTR1 could be a possible therapeutic target for gastric cancer. English Springer Verlag Activation of matrix metalloproteinase-9 (MMP-9) by neurotensin promotes cell invasion and migration through ERK pathway in gastric cancer Article 10.1007/s13277-015-3282-9 1 Tumor Biology, v.36, no.8, pp.6053 - 6062 Tumor Biology 36 8 6053 6062 N scie scopus 000360193800040 2-s2.0-84940435307 Oncology Oncology Article LUNG-CANCER EXPRESSION GROWTH RECEPTOR ADENOCARCINOMA METASTASIS MODULATION ESOPHAGEAL SECRETION MARKERS Neurotensin NTR1 ELISA MMP-9 Invasion ERK