Byeon, Seul Kee Kim, Jin Yong Lee, Ju Yong Chung, Bong Chul Seo, Hong Seog Moon, Myeong Hee 2024-01-20T06:33:00Z 2024-01-20T06:33:00Z 2021-09-05 2015-07-31 0021-9673 https://pubs.kist.re.kr/handle/201004/125212 This study demonstrated the performances of top-down and bottom-up approaches in lipidomic analysis of lipoproteins from rabbits raised under different metabolic conditions: healthy controls, carrageenan-induced inflammation, dehydration, high cholesterol (HC) diet, and highest cholesterol diet with inflammation (HCI). In the bottom-up approach, the high density lipoproteins (HDL) and the low density lipoproteins (LDL) were size-sorted and collected on a semi-preparative scale using a multiplexed hollow fiber flow field-flow fractionation (MxHF5), followed by nanoflow liquid chromatography-ESI-MS/MS (nLC-ESI-MS/MS) analysis of the lipids extracted from each lipoprotein fraction. In the top-down method, size-fractionated lipoproteins were directly infused to MS for quantitative analysis of targeted lipids using chip-type asymmetrical flow field-flow fractionation-electrospray ionization-tandem mass spectrometry (cAF4-ESI-MS/MS) in selected reaction monitoring (SRM) mode. The comprehensive bottom-up analysis yielded 122 and 104 lipids from HDL and LDL, respectively. Rabbits within the HC and HCI groups had lipid patterns that contrasted most substantially from those of controls, suggesting that HC diet significantly alters the lipid composition of lipoproteins. Among the identified lipids, 20 lipid species that exhibited large differences (>10-fold) were selected as targets for the top-down quantitative analysis in order to compare the results with those from the bottom-up method. Statistical comparison of the results from the two methods revealed that the results were not significantly different for most of the selected species, except for those species with only small differences in concentration between groups. The current study demonstrated that top-down lipid analysis using cAF4-ESI-MS/MS is a powerful high-speed analytical platform for targeted lipidomic analysis that does not require the extraction of lipids from blood samples. (C) 2015 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV CORONARY-ARTERY-DISEASE HEART-DISEASE CEREBROSPINAL-FLUID PLASMA PHOSPHOLIPIDS SEPARATION IDENTIFICATION RISK CHOLESTEROL SUBCLASSES Top-down and bottom-up lipidomic analysis of rabbit lipoproteins under different metabolic conditions using flow field-flow fractionation, nanoflow liquid chromatography and mass spectrometry Article 10.1016/j.chroma.2015.05.059 1 JOURNAL OF CHROMATOGRAPHY A, v.1405, pp.140 - 148 JOURNAL OF CHROMATOGRAPHY A 1405 140 148 scie scopus 000357753500014 2-s2.0-84937519018 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article CORONARY-ARTERY-DISEASE HEART-DISEASE CEREBROSPINAL-FLUID PLASMA PHOSPHOLIPIDS SEPARATION IDENTIFICATION RISK CHOLESTEROL SUBCLASSES Top-down lipidomics nLC-ESI-MS/MS Rabbit serum Lipoproteins High cholesterol diet Carrageenan