Ahn, Jang-Won Kim, Sunjik Na, Wooju Baek, Su-Jin Kim, Jeong-Hwan Min, Keehong Yeom, Jeonghun Kwak, Hoyun Jeong, Sunjoo Lee, Cheolju Kim, Seon-Young Choi, Cheol Yong 2024-01-20T06:33:07Z 2024-01-20T06:33:07Z 2021-09-05 2015-07-27 0305-1048 https://pubs.kist.re.kr/handle/201004/125218 DNA double-strand breaks (DSBs) are the most severe type of DNA damage and are primarily repaired by non-homologous end joining (NHEJ) and homologous recombination (HR) in the G1 and S/G2 phase, respectively. Although CtBP-interacting protein (CtIP) is crucial in DNA end resection during HR following DSBs, little is known about how CtIP levels increase in an S phase-specific manner. Here, we show that Serpine mRNA binding protein 1 (SERBP1) regulates CtIP expression at the translational level in S phase. In response to camptothecin-mediated DNA DSBs, CHK1 and RPA2 phosphorylation, which are hallmarks of HR activation, was abrogated in SERBP1-depleted cells. We identified CtIP mRNA as a binding target of SERBP1 using RNA immunoprecipitation-coupled RNA sequencing, and confirmed SERBP1 binding to CtIP mRNA in S phase. SERBP1 depletion resulted in reduction of polysome-associated CtIP mRNA and concomitant loss of CtIP expression in S phase. These effects were reversed by reconstituting cells with wild-type SERBP1, but not by SERBP1 Delta RGG, an RNA binding defective mutant, suggesting regulation of CtIP translation by SERBP1 association with CtIP mRNA. These results indicate that SERBP1 affects HR-mediated DNA repair in response to DNA DSBs by regulation of CtIP translation in S phase. English OXFORD UNIV PRESS RNA-BINDING PROTEIN CELL LUNG-CANCER END RESECTION DAMAGE RESPONSE OVARIAN-CANCER EXPRESSION INTERACTS PATHWAY CHOICE OVEREXPRESSION SERBP1 affects homologous recombination-mediated DNA repair by regulation of CtIP translation during S phase Article 10.1093/nar/gkv592 1 NUCLEIC ACIDS RESEARCH, v.43, no.13, pp.6321 - 6333 NUCLEIC ACIDS RESEARCH 43 13 6321 6333 scie scopus 000359776500017 2-s2.0-84939635662 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article RNA-BINDING PROTEIN CELL LUNG-CANCER END RESECTION DAMAGE RESPONSE OVARIAN-CANCER EXPRESSION INTERACTS PATHWAY CHOICE OVEREXPRESSION