Lee, Yong-Deok Cho, Hong-Jun Choi, Mi-Hwa Park, Hoyong Bang, Joona Lee, Sangyoup Kwon, Ick Chan Kim, Sehoon 2024-01-20T06:33:22Z 2024-01-20T06:33:22Z 2021-09-05 2015-07-10 0168-3659 https://pubs.kist.re.kr/handle/201004/125232 Methylene blue (MB), a water-soluble cationic dye widely used in the clinic, is known to photosensitize the generation of cytotoxic singlet oxygen efficiently, and thus, has attracted interest as a potential drug for photodynamic therapy (PDT). However, its use for the in vivo PDT of cancer has been limited due to the inherently poor cell/ tissue accumulation and low biological stability in the free molecular form. Here, we report a simple and biocompatible nanocomplex formulation ofMB (NanoMB) that is useful for in vivo locoregional cancer treatment by PDT. NanoMB particles were constructed through the self-assembly of clinically usable molecules (MB, fatty acid and a clinically approved polymer surfactant) directed by the dual (electrostatic and hydrophobic) interactions between the ternary constituents. The nanocomplexed MB showed greatly enhanced cell internalization while keeping the photosensitization efficiency as high as free MB, leading to distinctive phototoxicity toward cancer cells. When administered to human breast cancer xenograft mice by peritumoral injection, NanoMB was capable of facile penetration into the tumor followed by cancer cell accumulation, as examined in vivo and histologically with the near-infrared fluorescence signal of MB. The quintuple PDT treatment by a combination of peritumorally injected NanoMB and selective laser irradiation suppressed the tumor volume efficaciously, demonstrating potential of NanoMB-based PDT as a biocompatible and safe method for adjuvant locoregional cancer treatment. (C) 2015 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV NEGATIVE BREAST-CANCER METHYLENE-BLUE SINGLET OXYGEN QUANTUM YIELDS IN-VITRO NANOPARTICLE PLATFORMS AQUEOUS-SOLUTION GRAPHENE OXIDE IDENTIFICATION DELIVERY Directed molecular assembly into a biocompatible photosensitizing nanocomplex for locoregional photodynamic therapy Article 10.1016/j.jconrel.2015.04.011 1 JOURNAL OF CONTROLLED RELEASE, v.209, pp.12 - 19 JOURNAL OF CONTROLLED RELEASE 209 12 19 scie scopus 000355674300002 2-s2.0-84928499399 Chemistry, Multidisciplinary Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Article NEGATIVE BREAST-CANCER METHYLENE-BLUE SINGLET OXYGEN QUANTUM YIELDS IN-VITRO NANOPARTICLE PLATFORMS AQUEOUS-SOLUTION GRAPHENE OXIDE IDENTIFICATION DELIVERY Methylene blue Polymer nanoparticles Directed molecular assembly Photodynamic therapy MDA-MB-231 breast cancer cell