Ko, Hyuk Choi, Yong-Hwan Chang, Seo-Yoon Lee, Ga-Yeon Song, Hyun-Woo Chang, Young Wook Kang, Min-Jung Pyun, Jae-Chul 2024-01-20T06:34:04Z 2024-01-20T06:34:04Z 2021-09-05 2015-07 0014-3057 https://pubs.kist.re.kr/handle/201004/125269 In this work, we demonstrate that parylene-N can improve the immobilization efficiency of proteins through a UV-treatment. The UV-treatment can be carried out under atmospheric conditions at room temperature without the need for the gas control and vacuum conditions that are required for plasma-treatment. Additionally, the UV-treatment does not require the collision of high-energy gas molecules; the surface roughness of parylene-N is maintained throughout the modification process. To characterize the physical and chemical properties of the UV-treated parylene-N, contact angle (advancing and receding), grazing angle FT-IR spectroscopy (for thin films), and X-ray photoelectron spectroscopy (for depth profiling) measurements were carried out. The protein immobilization efficiency of the UV-treated parylene-N was estimated with SPA biosensors using proteins with different surface charges, and the long-term stability of the protein immobilization capabilities were also estimated as compared with plasma-treated parylene-N. (C) 2015 Elsevier Ltd. All rights reserved. English PERGAMON-ELSEVIER SCIENCE LTD AUTODISPLAYED Z-DOMAINS SPR BIOSENSOR ELECTROCHEMICAL ELISA ESCHERICHIA-COLI PM-IRRAS H FILM IMMUNOASSAY LAYER ELECTRODE ASSAY Surface modification of parylene-N with UV-treatment to enhance the protein immobilization Article 10.1016/j.eurpolymj.2015.04.016 1 EUROPEAN POLYMER JOURNAL, v.68, pp.36 - 46 EUROPEAN POLYMER JOURNAL 68 36 46 scie scopus 000357230800004 2-s2.0-84928720534 Polymer Science Polymer Science Article AUTODISPLAYED Z-DOMAINS SPR BIOSENSOR ELECTROCHEMICAL ELISA ESCHERICHIA-COLI PM-IRRAS H FILM IMMUNOASSAY LAYER ELECTRODE ASSAY Parylene-N Protein immobilization Surface modification Surface plasmon resonance UV Plasma