Lee, Kyung Min Min, Kyoungseon Choi, Okkyoung Kim, Ki-Yeon Woo, Han Min Kim, Yunje Han, Sung Ok Um, Youngsoon 2024-01-20T06:34:38Z 2024-01-20T06:34:38Z 2021-09-04 2015-07 0960-8524 https://pubs.kist.re.kr/handle/201004/125301 Lignocellulosic biomass is being preferred as a feedstock in the biorefinery, but lignocellulosic hydrolysate usually contains inhibitors against microbial fermentation. Among these inhibitors, phenolics are highly toxic to butyric acid-producing and butanol-producing Clostridium even at a low concentration. Herein, we developed an electrochemical polymerization method to detoxify phenolic compounds in lignocellulosic hydrolysate for efficient Clostridium fermentation. After the electrochemical detoxification for 10 h, 78%, 77%, 82%, and 94% of p-coumaric acid, ferulic acid, vanillin, and syringaldehyde were removed, respectively. Furthermore, 71% of total phenolics in rice straw hydrolysate were removed without any sugar-loss. Whereas the cell growth and metabolite production of Clostridium tyrobutyricum and Clostridium beijerinckii were completely inhibited in un-detoxified hydrolysate, those in detoxifying rice straw hydrolysate were recovered to 70-100% of the control cultures. The electrochemical detoxification method described herein provides an efficient strategy for producing butanol and butyric acid through Clostridium fermentation with lignocellulosic hydrolysate. (C) 2015 Elsevier Ltd. All rights reserved. English Elsevier BV Electrochemical detoxification of phenolic compounds in lignocellulosic hydrolysate for Clostridium fermentation Article 10.1016/j.biortech.2015.03.129 1 Bioresource Technology, v.187, pp.228 - 234 Bioresource Technology 187 228 234 N scie scopus 000353193200031 2-s2.0-84926338414 Agricultural Engineering Biotechnology & Applied Microbiology Energy & Fuels Agriculture Biotechnology & Applied Microbiology Energy & Fuels Article BUTANOL PRODUCTION ACID CONVERSION PRODUCTS GRAINS Electrochemical detoxification Phenolic compounds Clostridium fermentation Lignocellulosic hydrolysate