Kim, Myungsuk Park, Young Gyun Lee, Hee-Ju Lim, Sue Ji Nho, Chu Won 2024-01-20T07:00:38Z 2024-01-20T07:00:38Z 2021-09-05 2015-06-10 0021-8561 https://pubs.kist.re.kr/handle/201004/125332 This study investigated the effects of youngiaside A (YA), youngiaside C (YC), and Youngia denticulatum extract (YDE) on extrinsic aging and assessed its molecular mechanisms in UVB-irradiated HaCaT keratinocytes and human dermal fibroblasts (HDFs). The results showed that YA, YC, and YDE decreased matrix metalloproteinase (MMP) expression and production in HaCaT cell and HDFs and increased collagen expression and production in HDFs. In addition, YA, YC, and YDE significantly increased antioxidant enzyme expression, thereby down-regulating UVB-induced reactive oxygen species (ROS) production and ROS-induced mitogen-activated protein kinase (MAPK) and activator protein-1 (AP-1) signaling in HaCaT cells. Furthermore, YA, YC, and YDE reduced phosphorylation of I?Ba and IKK alpha/beta, blocked nuclear factor-kappa B (NF-kappa B) p65 nuclear translocation, and strongly suppressed pro-inflammatory mediators. Finally, YA, YC, and YDE augmented UVB-induced adenosine monophosphate activated protein kinase (AMPK) phosphorylation and YA and YC did not inhibit MMP-1 production in AMPK inhibitor or nuclear factor-erythroid 2-related factor-2 (Nrf2) siRNA-treated HaCaT cells. The results suggest that these compounds could be potential therapeutic agents for prevention and treatment of skin photoaging. English AMER CHEMICAL SOC MATRIX METALLOPROTEINASES OXIDATIVE DAMAGE KAPPA-B ULTRAVIOLET MECHANISMS PROTECTS GENE INDUCTION EXPOSURE EXTRACT Youngiasides A and C Isolated from Youngia denticulatum Inhibit UVB-Induced MMP Expression and Promote Type I Procollagen Production via Repression of MAPK/AP-1/NF-kappa B and Activation of AMPK/Nrf2 in HaCaT Cells and Human Dermal Fibroblasts Article 10.1021/acs.jafc.5b00467 1 JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.63, no.22, pp.5428 - 5438 JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 63 22 5428 5438 scie scopus 000356316800015 2-s2.0-84935845703 Agriculture, Multidisciplinary Chemistry, Applied Food Science & Technology Agriculture Chemistry Food Science & Technology Article MATRIX METALLOPROTEINASES OXIDATIVE DAMAGE KAPPA-B ULTRAVIOLET MECHANISMS PROTECTS GENE INDUCTION EXPOSURE EXTRACT youngiaside Youngia denticulatum UV MMPs type I procollagen