Yun, Ji Ho Park, Young Gyun Lee, Kyung-Mi Kim, Jungho Nho, Chu Won 2024-01-20T07:01:15Z 2024-01-20T07:01:15Z 2021-09-05 2015-06 1613-4125 https://pubs.kist.re.kr/handle/201004/125364 ScopeOctamer-binding transcription factor 4 (Oct4) is a key regulator of pluripotent embryonic stem cell maintenance. However, increasing evidence has suggested that Oct4 is also expressed in cancer stem cells (CSCs) and is associated with tumor progression and chemoresistance. Curcumin (CUR) is a widely used cancer chemopreventive agent, and it has been used to treat several diseases including cancers. Here, we investigated whether CUR-induced apoptotic cell death by inhibiting Oct4 levels and examining molecular mechanisms in NCCIT human embryonic carcinoma cells. Methods and resultsCUR significantly inhibited Oct4 transcription levels in a dose-dependent manner by dual luciferase experiment, also decreased mRNA and protein levels in NCCIT human embryonic carcinoma cells, which express high levels of endogenous Oct4. Interestingly, we found that CUR treatment increased apoptotic cell death including subG0/G1 contents, cleavage caspases, and pro-apoptotic protein, as confirmed with a series of loss-of-function experiments using Oct4 siRNA. Furthermore, CUR induced marked total level of glycogen synthase kinase 3 beta (GSK-3), resulting in an increase in apoptotic cell death, was evaluated using chemical inhibitor of GSK3-3. ConclusionThese data suggest that CUR induces apoptotic cell death through Oct4 inhibition and GSK-3 activation. Thus, CUR may be a useful cancer chemopreventive agent to suppress tumor progression or to improve chemoresistance by eliminating CSCs. English WILEY CANCER STEM-CELLS TRANSCRIPTION FACTOR COLORECTAL-CANCER BETA-CATENIN MESENCHYMAL TRANSITION DOWN-REGULATION SELF-RENEWAL EXPRESSION PATHWAY CHEMOPREVENTION Curcumin induces apoptotic cell death via Oct4 inhibition and GSK-3 activation in NCCIT cells Article 10.1002/mnfr.201400739 1 MOLECULAR NUTRITION & FOOD RESEARCH, v.59, no.6, pp.1053 - 1062 MOLECULAR NUTRITION & FOOD RESEARCH 59 6 1053 1062 scie scopus 000355745400004 2-s2.0-84930181383 Food Science & Technology Food Science & Technology Article CANCER STEM-CELLS TRANSCRIPTION FACTOR COLORECTAL-CANCER BETA-CATENIN MESENCHYMAL TRANSITION DOWN-REGULATION SELF-RENEWAL EXPRESSION PATHWAY CHEMOPREVENTION Apoptotic cell death Cancer chemoprevention Curcumin Oct4 Wnt/beta-catenin signaling