Son, Hyun-Hwa Kim, Shin Hye Moon, Ju-Yeon Chung, Bong Chul Park, Mi Jung Choi, Man Ho 2024-01-20T07:03:57Z 2024-01-20T07:03:57Z 2021-09-05 2015-05 0960-0760 https://pubs.kist.re.kr/handle/201004/125502 Quantitative sterol profiling in obese children and their clinical implications have not been fully investigated. The aim of study was to evaluate the metabolic changes in serum cholesterol and its precursors and metabolites, and their associations with clinical characteristics of childhood obesity. A total of 253 children aged 6-14 years (72 obese, 39 overweight, and 72 normal controls; 147 girls and 106 boys) were recruited. Anthropometric indices, body composition, and fasting total lipid profiles were determined. Serum concentrations of 20 sterols, as their free fraction, were analyzed through gas chromatography-mass spectrometry-based metabolite profiling. There were no significant differences in total- and LDL-cholesterols between groups. Serum levels of the main cholesterol precursors, lanosterol (P < 0.02) and lathosterol (P < 0.0001), were significantly higher in obese children. In addition, they showed positive correlations with waist to hip ratio, body fat percent, and body fat mass. The metabolic ratios of lanosterol and lathosterol to cholesterol were also elevated (P < 0.01 both), indicating the up-regulation of cholesterol biosynthesis with childhood obesity. In contrast, the absorption of plant sterols tended to show a compensatory decrease in obese children. Strong correlations between free cholesterol and total- and LDL-cholesterols were observed (r > 0.760, P < 0.001), while there was no correlation with HDL-cholesterols. The levels of total cholesteryl ester were closely associated with triglyceride (r = 0.763, P < 0.001). Quantitative results indicate that childhood obesity may increase cholesterol synthesis while maintaining overall cholesterol homeostasis. (C) 2015 Elsevier Ltd. All rights reserved. English PERGAMON-ELSEVIER SCIENCE LTD MICROSOMAL 3-HYDROXY-3-METHYLGLUTARYL INSULIN-RESISTANCE METABOLIC SYNDROME PLASMA ABSORPTION CHILDREN REFLECT MEN 24S-HYDROXYCHOLESTEROL ASSOCIATION Serum sterol profiling reveals increased cholesterol biosynthesis in childhood obesity Article 10.1016/j.jsbmb.2015.02.012 1 JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, v.149, pp.138 - 145 JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY 149 138 145 scie scopus 000352171400016 2-s2.0-84923924913 Biochemistry & Molecular Biology Endocrinology & Metabolism Biochemistry & Molecular Biology Endocrinology & Metabolism Article MICROSOMAL 3-HYDROXY-3-METHYLGLUTARYL INSULIN-RESISTANCE METABOLIC SYNDROME PLASMA ABSORPTION CHILDREN REFLECT MEN 24S-HYDROXYCHOLESTEROL ASSOCIATION Obesity Cholesterol Homeostasis Lathosterol Sterol profiling