Kim, Eun-Mi Eom, Jin-Hee Um, Youngsoon Kim, Yunje Woo, Han Min 2024-01-20T07:04:21Z 2024-01-20T07:04:21Z 2021-09-04 2015-05 0021-8561 https://pubs.kist.re.kr/handle/201004/125523 Myrcene, a monoterpene (C-10), has gathered attention as a starting material for high-value compounds, such as geraniol/linalool and (-)-menthol. Metabolic engineering has been successfully applied to produce monoterpenes, such as pinene and limonene, at high levels in microbial hosts. However, microbial synthesis of myrcene has not yet been reported. Thus, we metabolically engineered Escherichia coli for production of myrcene by introducing a heterologous mevalonate pathway and overexpressing tailoring enzymes, such as geranyl diphosphate synthase (GPPS) and myrcene synthase (MS). Although MSs have broad ranges of functionality for producing various monoterpenes, our engineered E. coli strains harboring MS from Quercus ilex L. produced only myrcene (1.67 +/- 0.029 mg/L). Subsequent engineering resulted in higher production of myrcene by optimizing the levels of GPPS in amino-acid-enriched (EZ-rich) defined medium, where glycerol as a carbon source was used. The production level of myrcene (58.19 +/- 12.13 mg/L) was enhanced by 34-fold using in situ two-phase extraction to eliminate cellular toxicity and the evaporation of myrcene. English American Chemical Society Microbial Synthesis of Myrcene by Metabolically Engineered Escherichia coil Article 10.1021/acs.jafc.5b01334 1 Journal of Agricultural and Food Chemistry, v.63, no.18, pp.4606 - 4612 Journal of Agricultural and Food Chemistry 63 18 4606 4612 N scie scopus 000354908800026 2-s2.0-84929376563 Agriculture, Multidisciplinary Chemistry, Applied Food Science & Technology Agriculture Chemistry Food Science & Technology Article FUNCTIONAL EXPRESSION ADVANCED BIOFUEL CDNA ISOLATION COLI MONOTERPENE SYNTHASE BIOSYNTHESIS GLYCEROL PINENE PRECURSOR mevalonate pathway metabolic engineering monoterpene myrcene