Shin, Miyoung Lee, Kyung-Eun Yang, Eun Gyeong Jeon, Hyesung Song, Hyun Kyu 2024-01-20T07:04:55Z 2024-01-20T07:04:55Z 2021-09-05 2015-04-13 0014-5793 https://pubs.kist.re.kr/handle/201004/125551 Phosphoprotein enriched in astrocytes of 15kDa (PEA-15) is known to sequester extracellular signal-regulated kinase (ERK) in the cytoplasm, inhibiting tumorigenesis of human breast cancer cells. Here, we describe how PEA-15 expression affects the dephosphorylation of epidermal growth factor receptor (EGFR) through endoplasmic reticulum (ER)-plasma membrane (PM) contacts in MDA-MB-468, triple-negative breast cancer (TNBC) cells. The increased intracellular calcium concentration resulting from increased cytoplasmic phosphorylated ERK facilitates movement of ER-anchored calcium sensors to the PM. The driving force of trans-localization of calcium-dependent proteins enhances the contact between the activated EGFR and ER-localized phosphatase, PTP1B. Consequently, our findings suggest a mechanism underneath the facilitation of EGFR dephosphorylation by cytoplasmic PEA-15 expression inside TNBC cells, which may be one of the dynamic mechanisms for down-regulation of activated EGFR in cancer cells. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. English WILEY GROWTH-FACTOR RECEPTOR PROTEIN-KINASE-C BREAST-CANCER ENDOPLASMIC-RETICULUM MEMBRANE CONTACTS MAP KINASE SITES PHOSPHOPROTEIN ASTROCYTES ACTIVATION PEA-15 facilitates EGFR dephosphorylation via ERK sequestration at increased ER-PM contacts in TNBC cells Article 10.1016/j.febslet.2015.03.009 1 FEBS LETTERS, v.589, no.9, pp.1033 - 1039 FEBS LETTERS 589 9 1033 1039 scie scopus 000351936500010 2-s2.0-84930983435 Biochemistry & Molecular Biology Biophysics Cell Biology Biochemistry & Molecular Biology Biophysics Cell Biology Article GROWTH-FACTOR RECEPTOR PROTEIN-KINASE-C BREAST-CANCER ENDOPLASMIC-RETICULUM MEMBRANE CONTACTS MAP KINASE SITES PHOSPHOPROTEIN ASTROCYTES ACTIVATION EGFR dephosphorylation ER-PM contact PEA-15 pERK1/2 PTP1B Triple-negative breast cancer cells