Kim, Su Hee Hur, Woojune Kim, Ji Eun Min, Hye Jeong Kim, Sukwha Min, Hye Sook Kim, Byeung Kyu Kim, Soo Hyun Choi, Tae Hyun Jung, Youngmee 2024-01-20T07:30:15Z 2024-01-20T07:30:15Z 2021-09-05 2015-04-01 1937-3341 https://pubs.kist.re.kr/handle/201004/125557 The number of patients requiring flat bone transplantation continues to increase worldwide. Cell transplantation has been successfully applied clinically; however, it causes another defect site and the time requirements to harvest cells and expand them are considerable. In this study, KLD12/KLD12-SP (KLD12+KLD12-substance P [SP]) was designed to mimic endogenous tissue-healing processes. The structures of KLD12, KLD12-SP, and KLD12/KLD12-SP were observed by transmission electron microscopy and circular dichroism spectra. KLD12/KLD12-SP nanofibers (5-10 nm) were created under physiological conditions by formation of a beta-sheet structure. The ability of mesenchymal stem cells (MSCs) to recruit KLD12/KLD12-SP was observed by using an in vivo fluorescence imaging system. Labeled human bone marrow stromal cells supplied via an intravenous injection were recruited to the scaffold containing KLD12/KLD12-SP. Polylactic acid/beta-tricalcium phosphate (PLA/beta-TCP) scaffolds filled with KLD12/KLD12-SP were applied to repair calvarial defects. The composite constructs (groups: defect, PLA/beta-TCP, PLA/beta-TCP/KLD12, and PLA/beta-TCP/KLD12/KLD12-SP) were implanted into rat defect sites. Bone tissue regeneration was evaluated by observing gross morphology by hematoxylin and eosin and Masson's trichrome staining at 12 and 24 weeks after surgery. Gross morphology showed that the defect site was filled with new tissue that was integrated with host tissue in the KLD12/KLD12-SP group. In addition, from the staining data, cells were recruited to the defect site and lacunae structures formed in the KLD12/KLD12-SP group. From these results, the PLA/beta-TCP+KLD12/KLD12-SP composite construct was considered for enhancement of bone tissue regeneration without cell transplantation. English MARY ANN LIEBERT, INC STEM-CELL RECRUITMENT STROMAL CELLS HOMING FACTOR HYDROGEL MATRIX ANGIOGENESIS OSTEOCALCIN ISCHEMIA PROTEIN Self-Assembling Peptide Nanofibers Coupled with Neuropeptide Substance P for Bone Tissue Engineering Article 10.1089/ten.tea.2014.0472 1 TISSUE ENGINEERING PART A, v.21, no.7-8, pp.1237 - 1246 TISSUE ENGINEERING PART A 21 7-8 1237 1246 scie scopus 000352613600006 2-s2.0-84927604325 Cell & Tissue Engineering Cell Biology Engineering, Biomedical Materials Science, Biomaterials Cell Biology Engineering Materials Science Article STEM-CELL RECRUITMENT STROMAL CELLS HOMING FACTOR HYDROGEL MATRIX ANGIOGENESIS OSTEOCALCIN ISCHEMIA PROTEIN