Lo, Shun Qiang Koh, Dawn X. P. Sng, Judy C. G. Augustine, George J. 2024-01-20T07:30:37Z 2024-01-20T07:30:37Z 2022-01-25 2015-04 2329-4248 https://pubs.kist.re.kr/handle/201004/125575 We describe an experimental approach that uses light to both control and detect neuronal activity in mouse barrel cortex slices: blue light patterned by a digital micromirror array system allowed us to photostimulate specific layers and columns, while a red-shifted voltage-sensitive dye was used to map out large-scale circuit activity. We demonstrate that such all-optical mapping can interrogate various circuits in somatosensory cortex by sequentially activating different layers and columns. Further, mapping in slices from whisker-deprived mice demonstrated that chronic sensory deprivation did not significantly alter feedforward inhibition driven by layer 5 pyramidal neurons. Further development of voltage-sensitive optical probes should allow this all-optical mapping approach to become an important and high-throughput tool for mapping circuit interactions in the brain. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. English SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS All-optical mapping of barrel cortex circuits based on simultaneous voltage-sensitive dye imaging and channelrhodopsin-mediated photostimulation Article 10.1117/1.NPh.2.2.021013 1 NEUROPHOTONICS, v.2, no.2 NEUROPHOTONICS 2 2 N scopus 000370538400016 2-s2.0-84978932762 Neurosciences Optics Neurosciences & Neurology Optics Article FUNCTIONAL INDEPENDENCE SYNAPTIC CONNECTIONS ELECTRICAL-ACTIVITY SENSORY RESPONSES ACTION-POTENTIALS CORTICAL BARRELS LAYER-IV BARRELS SOMATOSENSORY CORTEX PYRAMIDAL NEURONS IN-VIVO voltage-sensitive dye channelrhodopsin barrel cortex optogenetics