Thai, Hien Bao Dieu Yu, Jin Kyung Park, Yeon-Joon Ahn, Dae-Ro 2024-01-20T07:30:42Z 2024-01-20T07:30:42Z 2022-01-10 2015-04 0003-2654 https://pubs.kist.re.kr/handle/201004/125580 We described a dual turn-on probe sensitive to both acidity and basicity, which could be designed by connecting a fluorophore to a quencher via metal-ligand interaction. Atto488-labeled nitrilotriacetic acid and polyhistidine peptide were used as the fluorophore and the quencher, respectively, and linked to each other by coordination with a cobalt(II) ion. After preparation of the probe, the pH-sensitive dual turn-on property of the probe has been successfully observed upon responding to both acidity and basicity of the solution. The probe has been employed as a signal reporter in assays of pH-changing enzymes such as penicillinase generating acidity and adenosine deaminase generating basicity. Furthermore, the practical utility of the probe was also demonstrated by utilizing the probe in the discrimination of beta-lactamase-producing bacteria. English Royal Society of Chemistry SPECTRUM BETA-LACTAMASES CLAVULANIC ACID RAPID DETECTION ENTEROBACTERIACEAE DEAMINASE A dual-responsive pH-sensor and its potential as a universal probe for assays of pH-changing enzymes Article 10.1039/c4an01844c 1 Analyst, v.140, no.8, pp.2804 - 2809 Analyst 140 8 2804 2809 scie scopus 000352141800034 2-s2.0-84952877446 Chemistry, Analytical Chemistry Article SPECTRUM BETA-LACTAMASES CLAVULANIC ACID RAPID DETECTION ENTEROBACTERIACEAE DEAMINASE