Lee, Sun-Mi Jellison, Taylor Alper, Hal S. 2024-01-20T07:31:13Z 2024-01-20T07:31:13Z 2021-09-05 2015-04 1860-6768 https://pubs.kist.re.kr/handle/201004/125608 Complete utilization of all available carbon sources in lignocellulosic biomass still remains a challenge in engineering Saccharomyces cerevisiae. Even with efficient heterologous xylose catabolic pathways, S. cerevisiae is unable to utilize xylose in lignocellulosic biomass unless xylan is depolymerized to xylose. Here we demonstrate that a blended bioprospecting approach along with pathway engineering and evolutionary engineering can be used to improve xylan catabolism in S. cerevisiae. Specifically, we perform whole genome sequencing-based bioprospecting of a strain with remarkable pentose catabolic potential that we isolated and named Ustilago bevomyces. The heterologous expression of xylan catabolic genes enabled S. cerevisiae to grow on xylan as a single carbon source in minimal medium. A combination of bioprospecting and metabolic pathway evolution demonstrated that the xylan catabolic pathway could be further improved. Ultimately, engineering efforts were able to achieve xylan conversion into ethanol of up to 0.22 g/L on minimal medium compositions with xylan. This pathway provides a novel starting point for improving lignocellulosic conversion by yeast. English WILEY-V C H VERLAG GMBH 18S RIBOSOMAL-RNA XYLITOL DEHYDROGENASE XYLOSE ISOMERASE PICHIA-STIPITIS YEAST-STRAIN GENOME GENE EXPRESSION ETHANOL REDUCTASE Xylan catabolism is improved by blending bioprospecting and metabolic pathway engineering in Saccharomyces cerevisiae Article 10.1002/biot.201400622 1 BIOTECHNOLOGY JOURNAL, v.10, no.4, pp.575 - U283 BIOTECHNOLOGY JOURNAL 10 4 575 U283 scie scopus 000352636500009 2-s2.0-84926186926 Biochemical Research Methods Biotechnology & Applied Microbiology Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Article 18S RIBOSOMAL-RNA XYLITOL DEHYDROGENASE XYLOSE ISOMERASE PICHIA-STIPITIS YEAST-STRAIN GENOME GENE EXPRESSION ETHANOL REDUCTASE Bioprospecting Saccharomyces cerevisiae Xylan Xylanase Xylosidase