Murale, Dhiraj P. Hong, Seong Cheol Yun, JiHyeon Yoon, Chang No Lee, Jun-Seok 2024-01-20T07:31:28Z 2024-01-20T07:31:28Z 2021-09-04 2015-04 1359-7345 https://pubs.kist.re.kr/handle/201004/125622 Photo-crosslinking agents have emerged as critical tools to investigate protein-protein interactions in complex proteomes, but there are few photocrosslinkers available at the moment. Here, we report the first rational design of a photo-crosslinking BODIPY fluorophore (pcBD) and its biological application for biomolecule labeling. As a photo-sensitizing functional motif, an aryl ketone group was incorporated into the BODIPY fluorophore, and a series of proteins were labeled by pcBD compounds upon UV irradiation. In order to investigate protein-protein interactions in a protein mixture, amino-functionalized pcBD was prepared and covalently attached to a ubiquitin ligase binding peptide. Upon UV irradiation, we could successfully visualize the substrates in the total lysate. These results provided a proof of concept for spatially controllable tagging via photo-activation of the pcBD scaffold, and demonstrated its potential usage for in situ labeling applications. English ROYAL SOC CHEMISTRY LIVING CELLS Rational design of a photo-crosslinking BODIPY for in situ protein labeling Article 10.1039/c5cc01068c 1 CHEMICAL COMMUNICATIONS, v.51, no.30, pp.6643 - 6646 CHEMICAL COMMUNICATIONS 51 30 6643 6646 scie scopus 000352269000044 2-s2.0-84926324925 Chemistry, Multidisciplinary Chemistry Article LIVING CELLS fluorescence BODIPY protein labeling