Kim, Heesuk Lee, Keel Yong Ryu, Soo Ryeon Jung, Kwang-Hwan Ahn, Tae Kyu Lee, Yeonhee Kwon, Oh-Sun Park, Sung-Jin Parker, Kevin Kit Shin, Kwanwoo 2024-01-20T07:33:08Z 2024-01-20T07:33:08Z 2021-09-05 2015-03 2046-2069 https://pubs.kist.re.kr/handle/201004/125710 A novel method to fabricate transmembrane protein (TP) embedded lipid bilayers using microcontact printing and applying proteoliposomes to different types of substrates, has been developed. The electrostatic interaction between the negatively charged proteoliposome and the substrate, which had been positively functionalized by microcontact printing, allowed the formation of TP-embedded, patterned lipid bilayers. The positively charged amino functional group on the substrate did effectively attract the negatively charged vesicles, inducing them to be adsorbed and subsequently ruptured to form a giant mosaic lipid bilayer, resulting in an immobilized TP-embedded lipid layer precisely on the targeted patterns, which were backfilled with a zwitterionic lipid bilayer. The rapid and highly selective recognition of the charged liposomes was visualized, and the biological functions of the TPs in the lipid matrix were also observed. English ROYAL SOC CHEMISTRY SUPPORTED LIPID-BILAYERS GIANT UNILAMELLAR VESICLES POLYMER-CUSHIONED BILAYERS INDIUM-TIN-OXIDE PHOSPHOLIPID-BILAYERS RECONSTITUTION SURFACE DETERGENTS FUSION SPECTROSCOPY Charge-selective membrane protein patterning with proteoliposomes Article 10.1039/c4ra12088d 1 RSC ADVANCES, v.5, no.7, pp.5183 - 5191 RSC ADVANCES 5 7 5183 5191 scie scopus 000346733500063 2-s2.0-84919776071 Chemistry, Multidisciplinary Chemistry Article SUPPORTED LIPID-BILAYERS GIANT UNILAMELLAR VESICLES POLYMER-CUSHIONED BILAYERS INDIUM-TIN-OXIDE PHOSPHOLIPID-BILAYERS RECONSTITUTION SURFACE DETERGENTS FUSION SPECTROSCOPY