Kim, Jeong Ah Kim, Hong Nam Im, Sun-Kyoung Chung, Seok Kang, Ji Yoon Choi, Nakwon 2024-01-20T07:33:24Z 2024-01-20T07:33:24Z 2021-09-05 2015-03 1932-1058 https://pubs.kist.re.kr/handle/201004/125725 We present an engineered three-dimensional (3D) in vitro brain microvasculature system embedded within the bulk of a collagen matrix. To create a hydrogel template for the functional brain microvascular structure, we fabricated an array of microchannels made of collagen I using microneedles and a 3D printed frame. By culturing mouse brain endothelial cells (bEnd.3) on the luminal surface of cylindrical collagen microchannels, we reconstructed an array of brain microvasculature in vitro with circular cross-sections. We characterized the barrier function of our brain microvasculature by measuring transendothelial permeability of 40 kDa fluorescein isothiocyanate-dextran (Stoke's radius of similar to 4.5 nm), based on an analytical model. The transendothelial permeability decreased significantly over 3 weeks of culture. We also present the disruption of the barrier function with a hyperosmotic mannitol as well as a subsequent recovery over 4 days. Our brain microvasculature model in vitro, consisting of system-in-hydrogel combined with the widely emerging 3D printing technique, can serve as a useful tool not only for fundamental studies associated with blood-brain barrier in physiological and pathological settings but also for pharmaceutical applications. (C) 2015 AIP Publishing LLC. English AMER INST PHYSICS MICROFLUIDIC PLATFORM EXTRACELLULAR-MATRIX BARRIER TUMOR PERMEABILITY MICROVESSELS DIFFUSION MANNITOL GELS BBB Collagen-based brain microvasculature model in vitro using three-dimensional printed template Article 10.1063/1.4917508 1 BIOMICROFLUIDICS, v.9, no.2 BIOMICROFLUIDICS 9 2 scie scopus 000353829200023 2-s2.0-84927915647 Biochemical Research Methods Biophysics Nanoscience & Nanotechnology Physics, Fluids & Plasmas Biochemistry & Molecular Biology Biophysics Science & Technology - Other Topics Physics Article; Proceedings Paper MICROFLUIDIC PLATFORM EXTRACELLULAR-MATRIX BARRIER TUMOR PERMEABILITY MICROVESSELS DIFFUSION MANNITOL GELS BBB