Kim, Tae-Jin Joo, Chirlmin Seong, Jihye Vafabakhsh, Reza Botvinick, Elliot L. Berns, Michael W. Palmer, Amy E. Wang, Ning Ha, Taekjip Jakobsson, Eric Sun, Jie Wang, Yingxiao 2024-01-20T07:34:21Z 2024-01-20T07:34:21Z 2021-09-05 2015-02-10 2050-084X https://pubs.kist.re.kr/handle/201004/125769 It is unclear that how subcellular organelles respond to external mechanical stimuli. Here, we investigated the molecular mechanisms by which mechanical force regulates Ca2+ signaling at endoplasmic reticulum (ER) in human mesenchymal stem cells. Without extracellular Ca2+, ER Ca2+ release is the source of intracellular Ca2+ oscillations induced by laser- tweezer- traction at the plasma membrane, providing a model to study how mechanical stimuli can be transmitted deep inside the cell body. This ER Ca2+ release upon mechanical stimulation is mediated not only by the mechanical support of cytoskeleton and actomyosin contractility, but also by mechanosensitive Ca2+ permeable channels on the plasma membrane, specifically TRPM7. However, Ca2+ influx at the plasma membrane via mechanosensitive Ca2+ permeable channels is only mediated by the passive cytoskeletal structure but not active actomyosin contractility. Thus, active actomyosin contractility is essential for the response of ER to the external mechanical stimuli, distinct from the mechanical regulation at the plasma membrane. English ELIFE SCIENCES PUBLICATIONS LTD INTRACELLULAR CALCIUM-RELEASE MECHANOSENSITIVE ION CHANNELS MESENCHYMAL STEM-CELLS SMOOTH-MUSCLE-CELLS LIGHT-CHAIN KINASE T-LYMPHOMA CELLS NITRIC-OXIDE MOLECULAR-BASIS IP3 RECEPTORS LIVING CELLS Distinct mechanisms regulating mechanical force-induced Ca2+ signals at the plasma membrane and the ER in human MSCs Article 10.7554/eLife.04876 1 ELIFE, v.4 ELIFE 4 scie scopus 000349460800001 2-s2.0-84922879683 Biology Life Sciences & Biomedicine - Other Topics Article INTRACELLULAR CALCIUM-RELEASE MECHANOSENSITIVE ION CHANNELS MESENCHYMAL STEM-CELLS SMOOTH-MUSCLE-CELLS LIGHT-CHAIN KINASE T-LYMPHOMA CELLS NITRIC-OXIDE MOLECULAR-BASIS IP3 RECEPTORS LIVING CELLS