Lee, Eun Jung Lee, So Jin Kang, Yoon-Sik Ryu, Ju Hee Kwon, Koo Chul Jo, Eunji Yhee, Ji Young Kwon, Ick Chan Kim, Kwangmeyung Lee, Jeewon 2024-01-20T08:00:48Z 2024-01-20T08:00:48Z 2021-09-04 2015-02 1616-301X https://pubs.kist.re.kr/handle/201004/125837 Considering the problems of small interfering RNA (siRNA) delivery using traditional viral and nonviral vehicles, a new siRNA delivery system to enhance efficiency and safety needs to be developed. Here human ferritin-based proteinticles are genetically engineered to simultaneously display various functional peptides on the surface of proteinticles: cationic peptide to capture siRNA, tumor cell targeting and penetrating peptides, and enzymatically cleaved peptide to release siRNA inside tumor cell. In the in vitro treatment of poly-siRNA-proteinticle complex, both of the tumor cell targeting and penetrating peptides are important for efficient delivery of siRNA, and the red fluorescent protein (RFP) expression in RFP-expressing tumor cells is notably suppressed by the delivered siRNA with the complementary sequence to RFP mRNA. It seems that the human ferritin-based proteinticle is an efficient, stable, and safe tool for siRNA delivery, having a great potential for application to in vivo cancer treatment. The unique feature of proteinticles is that multiple and functional peptides can be simultaneously and evenly placed and also easily switched on the proteinticle surface through a simple genetic modification, which is likely to make proteinticles appropriate for targeted delivery of siRNA to a wide range of cancer cells. English WILEY-V C H VERLAG GMBH Engineered Proteinticles for Targeted Delivery of siRNA to Cancer Cells Article 10.1002/adfm.201403680 1 ADVANCED FUNCTIONAL MATERIALS, v.25, no.8, pp.1279 - 1286 ADVANCED FUNCTIONAL MATERIALS 25 8 1279 1286 N scie scopus 000349817200013 2-s2.0-85027932149 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Physics, Condensed Matter Chemistry Science & Technology - Other Topics Materials Science Physics Article SMALL INTERFERING RNAS IN-VIVO GENE-THERAPY TAT-PEPTIDE HEPATOCELLULAR-CARCINOMA TRANSDUCTION DOMAINS SYSTEMIC DELIVERY CELLULAR UPTAKE NANOPARTICLES THERAPEUTICS Gene silencing Proteinticles siRNA Surface engineering Targeted delivery