Lee, Sung-Woo Hyun, Kyung-A. Kim, Seung-Il Kang, Ji-Yoon Jung, Hyo-Il 2024-01-20T08:01:19Z 2024-01-20T08:01:19Z 2021-09-05 2015-01-16 0021-9673 https://pubs.kist.re.kr/handle/201004/125861 The isolation and characterization of circulating tumor cells (CTC) is of great importance in cancer diagnosis and prognosis. Highly sensitive detection of CTCs can be very difficult because they are extremely rare (i.e., 1-5 CTCs per 10(9) erythrocytes) in blood. Recently, various devices have been developed that exploit biochemical (affinity-based) and physical (size or density) methods. Antibody-based isolation has its own limitations, as the expression level of the epitopes for an antibody varies due to the heterogeneity of cancer cells. Harsh conditions associated with physical methods can cause the deformation and damage of CTCs during the isolation process. Here, we propose a microfluidic lateral flow filtration (mu-LaFF) chip in which lateral flow was combined with vertical flow into the filter to capture the CTCs gently. The CTCs experienced weak shear flow owing to the lateral flow and traveled alongside the filter channel until finally being captured. The vertical flow in the filter held the captured cells tightly and served as an exit for uncaptured hematological cells (white and red blood cells). From our mu-LaFF chip we obtained a high capture efficiency (95%) and purity (99%), minimizing any damage to the CTCs. Our mu-LaFF technology is expected to be useful in the diagnosis and prognosis of various cancers. (C) 2014 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV LABEL-FREE ISOLATION CANCER-PATIENTS BREAST-CANCER CAPTURE DEVICE CTCS Continuous enrichment of circulating tumor cells using a microfluidic lateral flow filtration chip Article 10.1016/j.chroma.2014.12.037 1 JOURNAL OF CHROMATOGRAPHY A, v.1377, pp.100 - 105 JOURNAL OF CHROMATOGRAPHY A 1377 100 105 scie scopus 000348556400011 2-s2.0-84920398964 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article LABEL-FREE ISOLATION CANCER-PATIENTS BREAST-CANCER CAPTURE DEVICE CTCS Circulating tumor cells(CTCs) Lateral flow filtration Microfluidics Enrichment