Bae, Nayoung Chung, Sungkwon Kim, Hee Ju Cha, Jin Wook Oh, Hyungun Gu, Ming-Yao Oh, Myung Sook Yang, Hyun Ok 2024-01-20T08:01:22Z 2024-01-20T08:01:22Z 2021-09-05 2015-01-15 0378-8741 https://pubs.kist.re.kr/handle/201004/125864 Aim of the study: Previous studies in our laboratory revealed the neuroprotective effect of modified Yeoldahanso-tang (MYH) in models of Parkinson's disease (PD). In this study, we investigated another traditional Korean herbal formula, modified Chungsimyeolda-tang (termed DG), as a potential treatment for PD. Chungsimyeolda-tang has been used in Korea to treat cerebrovascular diseases, such as stroke. Here, we verify the neuroprotective and autophagy-inducing effects of DG to evaluate any potential anti-parkinsonian properties. Materials and methods: 1-Methyl-4-phenylpyridinium (MPP+) and rotenone were used to induce cytotoxicity in nerve growth factor (NGF)-differentiated rat pheochromocytoma (PC12) cells. Cell viability was measured using an MTT assay. Induction of autophagy by DG in NGF-differentiated PC12 cells was measured using an immunoblotting assay with an LC3 antibody. The proteasomal inhibitor lactacystin was used to induce ubiquitin-proteasome system (UPS) dysfunction in NGF-differentiated PC12 cells. DG-mediated clearance of aggregated proteins was measured using an immunoblotting assay with a ubiquitin antibody. Results and conclusions: Our findings indicate that DG robustly protects NGF-differentiated PC12 cells against the neurotoxic effects of MPP+ and rotenone in an in vitro model. Furthermore, DG protects NGFdifferentiated PC12 cells against lactacystin-induced cell death. This effect is partially mediated by an increased autophagy associated with the enhanced degradation of aggregated proteins. This study suggests that DG is an attractive candidate drug for inducing autophagy and, therefore, may represent a promising strategy to prevent diseases associated with misfolded/aggregated proteins in various neurodegenerative disorders, including Parkinson's disease. (C) 2014 Elsevier Ireland Ltd. All rights reserved. English ELSEVIER IRELAND LTD CHAPERONE-MEDIATED AUTOPHAGY POLYGALA-TENUIFOLIA WILLD ALPHA-SYNUCLEIN 1-METHYL-4-PHENYL-1,2,3,6-TETRAHYDROPYRIDINE MPTP CELL-DEATH PROTEASOME INHIBITION NEUROTOXIN BRAIN HEPATOCYTES Neuroprotective effect of modified Chungsimyeolda-tang, a traditional Korean herbal formula, via autophagy induction in models of Parkinson's disease Article 10.1016/j.jep.2014.11.007 1 JOURNAL OF ETHNOPHARMACOLOGY, v.159, pp.93 - 101 JOURNAL OF ETHNOPHARMACOLOGY 159 93 101 scie scopus 000348271500011 2-s2.0-84912571424 Plant Sciences Chemistry, Medicinal Integrative & Complementary Medicine Pharmacology & Pharmacy Plant Sciences Pharmacology & Pharmacy Integrative & Complementary Medicine Article CHAPERONE-MEDIATED AUTOPHAGY POLYGALA-TENUIFOLIA WILLD ALPHA-SYNUCLEIN 1-METHYL-4-PHENYL-1,2,3,6-TETRAHYDROPYRIDINE MPTP CELL-DEATH PROTEASOME INHIBITION NEUROTOXIN BRAIN HEPATOCYTES DG Parkinson&apos s disease Neuroprotection Autophagy Lactacystin PC12 cells