Chung, Seung Woo Bae, Sang Mun Lee, Myungjin Al-Hilal, Taslim A. Lee, Chang Kyung Kim, Jeong Kon Kim, In-San Kim, Sang Yoon Byun, Youngro 2024-01-20T08:02:13Z 2024-01-20T08:02:13Z 2022-01-10 2015-01 0142-9612 https://pubs.kist.re.kr/handle/201004/125907 Despite the therapeutic benefits of the angiogenesis inhibitors shown in the clinics, they have encountered an unexpected limitation by the occurrence of acquired resistance. Although the mechanism of the resistance is not clear so far, the upregulation of alternative angiogenic pathways and stabilization of endothelium by mural cells were reported to be responsible. Therefore, blocking multiple angiogenic pathways that are crucial in tumor angiogenesis has been highlighted to overcome such limitations. To develop an angiogenesis inhibitor that could block multiple angiogenic factors, heparin is an excellent lead compound since wide array of angiogenic factors are heparin-binding proteins. In previous study, we reported a heparin-derived angiogenesis inhibitor, LHT7, as a potent angiogenesis inhibitor and showed that it blocked VEGF signaling pathway. Here we show that LHT7 could block the fibroblast growth factor 2 (FGF2) and platelet-derived growth factor B (PDGF-B) in addition to VEGF. Simultaneous blockade of these angiogenic factors resulted in inhibition of multiple stages of the angiogenic process, including initial angiogenic response to maturation of the endothelium by pericyte coverage in vitro. In addition, the treatment of LHT7 in vivo did not show any sign of vascular normalization and directly led to decreased blood perfusion throughout the tumor. Our findings show that LHT7 could effectively inhibit tumor angiogenesis by blocking multiple stages of the angiogenesis, and could potentially be used to overcome the resistance. (C) 2014 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD ENDOTHELIAL GROWTH-FACTOR ANTIANGIOGENIC THERAPY TUMOR ANGIOGENESIS KINASE INHIBITORS DRUG-RESISTANCE IN-VITRO CANCER PERICYTES CELLS EXPRESSION LHT7, a chemically modified heparin, inhibits multiple stages of angiogenesis by blocking VEGF, FGF2 and PDGF-B signaling pathways Article 10.1016/j.biomaterials.2014.10.004 1 BIOMATERIALS, v.37, pp.271 - 278 BIOMATERIALS 37 271 278 scie scopus 000346541100026 2-s2.0-84922291857 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article ENDOTHELIAL GROWTH-FACTOR ANTIANGIOGENIC THERAPY TUMOR ANGIOGENESIS KINASE INHIBITORS DRUG-RESISTANCE IN-VITRO CANCER PERICYTES CELLS EXPRESSION Heparin Angiogenesis VEGF FGF2 PDGF-B