Park, In-Su Kang, Jo A. Kang, Jungmi Rhie, Jong-Won Kim, Sang-Heon 2024-01-20T08:04:56Z 2024-01-20T08:04:56Z 2021-09-02 2014-12 1932-8486 https://pubs.kist.re.kr/handle/201004/126050 We investigated whether transplantation of three-dimensional cell masses (3DCM) of human adipose-derived stromal cells (hASCs) cultured on a basic fibroblast growth factor-immobilized substrate improved hind limb functional recovery by stimulating angiogenesis in an immune-competent rat ischemic limb model. In vitro experiments confirmed that cells within 3DCMs differentiate toward the endothelial lineage one day after culture in normal medium. The therapeutic effect of 3DCMs was evaluated by transplanting hASCs, phosphate-buffered saline alone, and the 3DCM into rat ischemic hind limbs. Blood flow was enhanced in the ischemic hind limb in the 3DCM-injected group compared with the other groups. The ratio of human nuclear antigen (HNA) and hVEGF-positive cells was significantly higher in the 3DCM-injected group compared to hASC-injected group. Human VEGF was observed in most HNA-positive cells. Many hCD31 and hSMA-positive cells were observed in vessel-like structures in the 3DCM-injected group. The 3DCM transplantation improved cell retention and angiogenic effects compared with ASC transplantation. These findings suggest that transplantation of 3DCMs may be an effective stem cell therapy for hind limb ischemia. Anat Rec, 297:2289-2298, 2014. (c) 2014 Wiley Periodicals, Inc. English WILEY CRITICAL LIMB ISCHEMIA FIBROBLAST-GROWTH-FACTOR MESENCHYMAL STEM-CELLS DIFFERENTIATION ANGIOGENESIS DISEASE TISSUE MODEL Therapeutic Effect of Human Adipose-Derived Stromal Cells Cluster in Rat Hind-Limb Ischemia Article 10.1002/ar.22961 1 ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY, v.297, no.12, pp.2289 - 2298 ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY 297 12 2289 2298 scie scopus 000345299700008 2-s2.0-84917738420 Anatomy & Morphology Anatomy & Morphology Article CRITICAL LIMB ISCHEMIA FIBROBLAST-GROWTH-FACTOR MESENCHYMAL STEM-CELLS DIFFERENTIATION ANGIOGENESIS DISEASE TISSUE MODEL adipose-derived stem cell angiogenesis endothelial differentiation hypoxic induction three-dimensional cell mass FGF2-immobilized substrate