Lee, Soo Hyun Park, Sungha Kim, Han-Soo Jung, Byung Hwa 2024-01-20T08:30:14Z 2024-01-20T08:30:14Z 2021-09-02 2014-12 1573-3882 https://pubs.kist.re.kr/handle/201004/126052 Aging is a multifaceted process involving the accumulation of diverse deleterious changes in biological systems over time, so significant alterations in cellular metabolism are detected throughout aging. In the present study, the metabolic processes relevant to the normal aging process were investigated via non-targeted metabolomics using liquid chromatography-mass spectrometry. To exclude physiological and environmental differences, the metabolic profiles and the relevant metabolic pathways were analyzed in plasma from two separate study groups comprising two distinctly aged cohorts of healthy individuals, the elderly and the younger. The first group was recruited from an urban hospital, and the second group was recruited from a rural community. Alterations in fatty acid beta-oxidation, glycerophospholipid metabolism, and sphingolipid metabolism were identified as significant metabolic pathways relevant to normal aging. It was also found that sphingosine in sphingolipid metabolism, long-chain acylcarnitines in beta-oxidation, and lysophosphatidylcholines (LysoPCs) in glycerophospholipid metabolism could be critical candidate metabolites in the aging process. These results suggest that the metabolic profile of the healthiest individuals could be associated with the normal function of mitochondria, the primary organelle of redox homeostasis, as indicated by their low acylcarnitine to l-carnitine ratio and low levels of LysoPCs and sphingosine in plasma. The present study provides a critical contribution to the entire picture of the aging process. English SPRINGER LIQUID-CHROMATOGRAPHY LIPID-PEROXIDATION MEMBRANE FLUIDITY OXIDATIVE STRESS HUMAN URINE HPLC-MS AGE LYSOPHOSPHATIDYLCHOLINE CARNITINE ACYLCARNITINES Metabolomic approaches to the normal aging process Article 10.1007/s11306-014-0663-9 1 METABOLOMICS, v.10, no.6, pp.1268 - 1292 METABOLOMICS 10 6 1268 1292 scie scopus 000344555300021 2-s2.0-84939879766 Endocrinology & Metabolism Endocrinology & Metabolism Article LIQUID-CHROMATOGRAPHY LIPID-PEROXIDATION MEMBRANE FLUIDITY OXIDATIVE STRESS HUMAN URINE HPLC-MS AGE LYSOPHOSPHATIDYLCHOLINE CARNITINE ACYLCARNITINES Metabolomics Aging UPLC-QTOF-MS Acylcarnitines Lysophospholipids Sphingolipid metabolism