Subbiah, Ramesh Du, Ping Van, Se Young Suhaeri, Muhammad Hwang, Mintai P. Lee, Kangwon Park, Kwideok 2024-01-20T08:30:20Z 2024-01-20T08:30:20Z 2021-09-02 2014-12 1748-6041 https://pubs.kist.re.kr/handle/201004/126057 An artificial matrix (Fn-Tigra), consisting of graphene oxide (GO) and fibronectin (Fn), is developed on pure titanium (Ti) substrates via an electrodropping technique assisted with a custom-made coaxial needle. The morphology and topography of the resulting artificial matrix is orderly aligned and composed of porous microcavities. In addition, Fn is homogenously distributed and firmly bound onto GO as determined via immunofluorescence and elemental mapping, respectively. The artificial matrix is moderately hydrophobic (63.7 degrees), and exhibits an average roughness of 546 nm and a Young's modulus (E) of approximately 4.8 GPa. The biocompatibility, cellular behavior, and osteogenic potential of preosteoblasts on Fn-Tigra are compared to those of cells cultured on Ti and Ti-GO (Tigra). Cell proliferation and viability are significantly higher on Fn-Tigra and Tigra than that of cells grown on Ti. Focal adhesion molecule (vinculin) expression is highly activated at the central and peripheral area of preosteoblasts when cultured on Fn-Tigra. Furthermore, we demonstrate enhanced in vitro osteogenic differentiation of preosteoblasts cultured on Fn-Tigra over those cultured on bare Ti, as determined via Alizarin red and von Kossa staining, and the analysis of osteocalcin, type I collagen, alkaline phosphatase activity, and calcium contents. Finally, we investigate the biophysical and biomechanical properties of the cells using AFM. While the height and roughness of preosteoblasts increased with time, cell surface area decreased during in vitro osteogenesis over 2 weeks. In addition, the E of cells cultured on Tigra and Fn-Tigra increase in a statistically significant and time-dependent manner by 30%, while those cultured on bare Ti retain a relatively consistent E. In summary, we engineer a biocompatible artificial matrix (Fn-Tigra) capable of osteogenic induction and consequently demonstrate its potential in bone tissue engineering applications. English IOP PUBLISHING LTD MESENCHYMAL STEM-CELLS ATOMIC-FORCE MICROSCOPY DIFFERENTIATION ADHESION ADSORPTION SCAFFOLDS TITANIUM PERFORMANCE SURFACES COLLAGEN Fibronectin-tethered graphene oxide as an artificial matrix for osteogenesis Article 10.1088/1748-6041/9/6/065003 1 BIOMEDICAL MATERIALS, v.9, no.6 BIOMEDICAL MATERIALS 9 6 scie scopus 000345615600004 2-s2.0-84914109464 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article MESENCHYMAL STEM-CELLS ATOMIC-FORCE MICROSCOPY DIFFERENTIATION ADHESION ADSORPTION SCAFFOLDS TITANIUM PERFORMANCE SURFACES COLLAGEN graphene oxide fibronectin titanium electrodropping system osteogenic differentiation atomic force microscopy