Karim, Md. Nurul Lee, Ji Eun Lee, Hye Jin 2024-01-20T08:31:30Z 2024-01-20T08:31:30Z 2021-09-02 2014-11-15 0956-5663 https://pubs.kist.re.kr/handle/201004/126115 A novel amperometric biosensor for catechol was developed using the layer-by-layer (LbL) self-assembly of positively charged hexadecyltrimethylammonium stabilized gold nanocubes (AuNCs), negatively charged poly(sodium 4-styrenesulfonate) and tyrosinase on a screen printed carbon electrode (SPCE). A carboxylic acid terminated alkanethiol assembled on electrochemically deposited Au nanoparticles on a SPCE was used as a platform for LbL assembly. Each SPCE sensor surface was terminated with tyrosinase and the electrocatalytic response due to the tyrosinase reaction with catechol was measured using cyclic voltammetry and square wave voltammetry (SWV). The effect of introducing AuNCs into the LbL assembly to further enhance the catechol detection performance was then investigated by comparing the SWV results to those from biosensors created using both the tyrosinase modified LbL assembly in the absence of NCs and the covalent attachment of tyrosinase. A wide dynamic range from 10 nM to 80 mu M of catechol with an excellent sensitivity of 13.72 A/M and a detection limit of 0.4 nM were both achieved alongside a good selectivity and reproducibility for the AuNC-modified electrodes. As a demonstration, the optimized biosensor design was applied to determine catechol concentrations in tea samples. (C) 2014 Elsevier B.V. All rights reserved. English ELSEVIER ADVANCED TECHNOLOGY NANOPARTICLES IMMOBILIZATION Amperometric detection of catechol using tyrosinase modified electrodes enhanced by the layer-by-layer assembly of gold nanocubes and polyelectrolytes Article 10.1016/j.bios.2014.05.011 1 BIOSENSORS & BIOELECTRONICS, v.61, pp.147 - 151 BIOSENSORS & BIOELECTRONICS 61 147 151 scie scopus 000339692500022 2-s2.0-84901317333 Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics Article NANOPARTICLES IMMOBILIZATION Amperometric biosensor Catechol Gold nanocubes Tyrosinase Layer-by-layer Screen printed carbon electrode