Shin, Eun Sil
Hwang, Onyou
Hwang, Yu-Shik
Suh, Jun-Kyo Francis
Chun, Young Il
Jeon, Sang Ryong
2024-01-20T08:32:07Z
2024-01-20T08:32:07Z
2022-01-25
2014-11
2005-3711
https://pubs.kist.re.kr/handle/201004/126146
Objective : Neural tissue transplantation has been a promising strategy for the treatment of Parkinson's disease (PD). However, transplantation has the disadvantages of low-cell survival and/or development of dyskinesia. Transplantation of cell aggregates has the potential to overcome these problems, because the cells can extend their axons into the host brain and establish synaptic connections with host neurons. In this present study, aggregates of human brain-derived neural stem cells (HB-NSC) were transplanted into a PD animal model and compared to previous report on transplantation of single-cell suspensions. Methods : Rats received an injection of 6-OHDA into the right medial forebrain bundle to generate the PD model and followed by injections of PBS only, or HB-NSC aggregates in PBS into the ipsilateral striatum. Behavioral tests, multitracer (2-deoxy-2-[F-18]-fluoro-D-glucose ([F-18]-FDG) and [F-18]-N-(3-fluoropropyl)-2-carbomethoxy-3-(4-iodophenyl)nortropane (F-18]-FP-CIT) microPET scans, as well as immunohistochemical (IHC) and immunofluorescent (IF) staining were conducted to evaluate the results. Results : The stepping test showed significant improvement of contralateral forelimb control in the HB-NSC group from 6-10 weeks compared to the control group (p<0.05). [F-18]-FP-CIT microPET at 10 weeks posttransplantation demonstrated a significant increase in uptake in the HB-NSC group compared to pretransplantation (p<0.05). In IHC and IF staining, tyrosine hydroxylase and human beta 2 microglobulin (a human cell marker) positive cells were visualized at the transplant site. Conclusion : These results suggest that the HB-NSC aggregates can survive in the striatum and exert therapeutic effects in a PD model by secreting dopamine.
English
KOREAN NEUROSURGICAL SOC
Enhanced Efficacy of Human Brain-Derived Neural Stem Cells by Transplantation of Cell Aggregates in a Rat Model of Parkinson's Disease
Article
10.3340/jkns.2014.56.5.383
1
JOURNAL OF KOREAN NEUROSURGICAL SOCIETY, v.56, no.5, pp.383 - 389
JOURNAL OF KOREAN NEUROSURGICAL SOCIETY
56
5
383
389
N
scie
scopus
kci
ART001954888
000346550600003
2-s2.0-84919415123
Clinical Neurology
Surgery
Neurosciences & Neurology
Surgery
Article
MULTIPLE SYSTEM ATROPHY
SUBSTANTIA-NIGRA
DOPAMINE
DYSKINESIAS
DYSFUNCTION
MICROPET
THERAPY
Parkinson&apos
s disease
Cell transplantation
Human brain-derived neural stem cells
Cell aggregates
Rat model
[F-18]-FP-CIT microPET