Oh, Ju-Hwan Kim, Byoung Chan Lee, Jae-Seung 2024-01-20T08:32:39Z 2024-01-20T08:32:39Z 2021-09-02 2014-11 1618-2642 https://pubs.kist.re.kr/handle/201004/126174 We have developed a highly sensitive and selective colorimetric method for detection of acetylcholine (ACh), using a tandem enzymatic reaction for biological target recognition and silver nanoplates (AgNPLs) for optical signal generation. The ACh molecules are enzymatically hydrolyzed and oxidized into betaine and hydrogen peroxide, the latter of which chemically oxidizes the AgNPLs to generate the "turn-on" signal. To optimize detection sensitivity, the chemical and biological properties of the detection mixtures containing the enzymes, ACh, and AgNPLs were thoroughly investigated with respect to component concentrations and reaction temperatures; a maximum sensitivity of 500 nM for colorimetric detection of ACh was achieved. We further directly compared the signaling profiles of (1) novel nanostructured and (2) conventional molecular chromogens, improving our understanding of the factors that should be considered when designing a detection system. English SPRINGER HEIDELBERG GOLD NANOPARTICLES SILVER NANOPARTICLES HYDROGEN-PEROXIDE OPTICAL-DETECTION AU NANOPARTICLES O-DIANISIDINE DNA DETECTION NANORODS GLUCOSE CHOLINE Colorimetric detection of acetylcholine with plasmonic nanomaterials signaling Article 10.1007/s00216-014-8199-4 1 ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v.406, no.29, pp.7591 - 7600 ANALYTICAL AND BIOANALYTICAL CHEMISTRY 406 29 7591 7600 scie scopus 000344860300018 2-s2.0-84925541578 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article GOLD NANOPARTICLES SILVER NANOPARTICLES HYDROGEN-PEROXIDE OPTICAL-DETECTION AU NANOPARTICLES O-DIANISIDINE DNA DETECTION NANORODS GLUCOSE CHOLINE Nanoparticles/nanotechnology Biosensors Optical sensors Enzymes