Kim, Choong Park, Juyoung Kang, Ji Yoon 2024-01-20T08:32:52Z 2024-01-20T08:32:52Z 2021-09-02 2014-11 1932-1058 https://pubs.kist.re.kr/handle/201004/126186 Cell encapsulation technology is a promising strategy applicable to tissue engineering and cell therapy. Many advanced microencapsulation chips that function via multiple syringe pumps have been developed to generate monodisperse hydrogel beads encapsulating cells. However, their operation is difficult and only trained microfluidic engineers can use them with dexterity. Hence, we propose a microfluidic manifold system, driven by a single syringe pump, which can enable the setup of automated flow sequences and generate highly mono-disperse alginate beads by minimizing disturbances to the pump pressure. The encapsulation of P19 mouse embryonic carcinoma cells and embryonic body formation are demonstrated to prove the efficiency of the proposed system. (C) 2014 AIP Publishing LLC. English AMER INST PHYSICS EMBRYONIC STEM-CELLS DEVICE MICROBEADS DELIVERY CULTURE BODIES LIVE A microfluidic manifold with a single pump system to generate highly mono-disperse alginate beads for cell encapsulation Article 10.1063/1.4902943 1 BIOMICROFLUIDICS, v.8, no.6 BIOMICROFLUIDICS 8 6 scie scopus 000347160400033 2-s2.0-84916618769 Biochemical Research Methods Biophysics Nanoscience & Nanotechnology Physics, Fluids & Plasmas Biochemistry & Molecular Biology Biophysics Science & Technology - Other Topics Physics Article EMBRYONIC STEM-CELLS DEVICE MICROBEADS DELIVERY CULTURE BODIES LIVE encapsulation alginate manifold