Choi, Okkyoung Kim, Taeyeon Woo, Han Min Um, Youngsoon 2024-01-20T08:32:53Z 2024-01-20T08:32:53Z 2021-09-02 2014-11 2045-2322 https://pubs.kist.re.kr/handle/201004/126187 Although microbes directly accepting electrons from a cathode have been applied for CO2 reduction to produce multicarbon-compounds, a high electron demand and low product concentration are critical limitations. Alternatively, the utilization of electrons as a co-reducing power during fermentation has been attempted, but there must be exogenous mediators due to the lack of an electroactive heterotroph. Here, we show that Clostridium pasteurianum DSM 525 simultaneously utilizes both cathode and substrate as electron donors through direct electron transfer. In a cathode compartment poised at +0.045 V vs. SHE, a metabolic shift in C. pasteurianum occurs toward NADH-consuming metabolite production such as butanol from glucose (20% shift in terms of NADH consumption) and 1,3-propandiol from glycerol (21% shift in terms of NADH consumption). Notably, a small amount of electron uptake significantly induces NADH-consuming pathways over the stoichiometric contribution of the electrons as reducing equivalents. Our results demonstrate a previously unknown electroactivity and metabolic shift in the biochemical-producing heterotroph, opening up the possibility of efficient and enhanced production of electron-dense metabolites using electricity. English Nature Publishing Group Electricity-driven metabolic shift through direct electron uptake by electroactive heterotroph Clostridium pasteurianum Article 10.1038/srep06961 1 Scientific Reports, v.4 Scientific Reports 4 Y scie scopus 000344385100013 2-s2.0-84923349564 Multidisciplinary Sciences Science & Technology - Other Topics Article MICROBIAL FUEL-CELLS FERMENTATION CARBON SHEWANELLA GLYCEROL BUTANOL