Kim, Jo-Il Park, Jong-Min Noh, Joo-Yoon Kang, Min-Jung Pyun, Jae-Chul 2024-01-20T08:33:03Z 2024-01-20T08:33:03Z 2021-09-02 2014-11 0951-4198 https://pubs.kist.re.kr/handle/201004/126196 RATIONALE: In matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS), volatile small molecules have been nearly impossible to analyze because (1) such molecules evaporate under drying and vacuum conditions and (2) the organic matrix creates matrix peaks in the low mass-to-charge (m/z) range (m/z <500). In this work, the analysis of volatile small molecules using MALDI-TOFMS was realized using (1) a parylene-matrix chip to eliminate the matrix peaks of the organic matrix and (2) graphene for the effective adsorption of the small volatile molecules. METHODSThe parylene-matrix chip was produced by deposition of a partially porous parylene-N thin film on a dried organic matrix array. The sample solution of volatile small molecules was mixed with the graphene and then placed on the parylene-matrix chip for MALDI-TOFMS. Analogs of chemical agents called dimethyl methyl phosphonate (DMMP) and 2-chloroethylethylsulfide (CEES) were used as model compounds for the small volatile molecules, and the sensing parameters were estimated, such as the limit of detection (LOD) and the detection range. RESULTSMALDI-TOFMS based on the parylene-matrix chip and graphene as the adsorbent could achieve a LOD of approximately 1ppb in the detection range of 1ppm - 1ppb for the highly volatile DMMP and CEES. CONCLUSIONSThe parylene-matrix chip with graphene can be applied for the detection of volatile small molecule analytes in the m/z ratio range of small molecules (m/z <500) using graphene as an effective adsorbent. Copyright (c) 2014 John Wiley & Sons, Ltd. English John Wiley & Sons Inc. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of small volatile molecules using a parylene-matrix chip Article 10.1002/rcm.7025 1 Rapid Communications in Mass Spectrometry, v.28, no.21, pp.2301 - 2306 Rapid Communications in Mass Spectrometry 28 21 2301 2306 N scie scopus 000342898700008 2-s2.0-84925584697 Biochemical Research Methods Chemistry, Analytical Spectroscopy Biochemistry & Molecular Biology Chemistry Spectroscopy Article WARFARE AGENT SIMULANTS SOLID-PHASE EXTRACTION WASTE-WATER TREATMENT MALDI-TOF MS CARBON NANOTUBES QUANTITATIVE-ANALYSIS POROUS SILICON GRAPHENE ADSORBENT DESORPTION MALDI-TOF MS Volatile Small Molecules Analysis Chip Nano Matrix