Shin, Jihye Kim, Hye-Jung Kim, Gamin Song, Meiying Woo, Se Joon Lee, Seung-Taek Kim, Hoguen Lee, Cheolju 2024-01-20T08:33:11Z 2024-01-20T08:33:11Z 2021-09-02 2014-11 1535-3893 https://pubs.kist.re.kr/handle/201004/126203 To discover serological colorectal cancer (CRC) markers, we analyzed cell line secretome to gather proteins of higher potential to be secreted from tissues into circulation. A total of 898 human proteins were identified, of which 62.2% were predicted to be released or shed from cells. The identified proteins were compared with tissue proteomes to find candidate proteins whose expressions were elevated in tumor tissues compared with normal tissues as revealed by (i) quantitative proteomic analysis based on cICAT and mTRAQ or (ii) data mining of immunohistochemical images piled in Human Protein Atlas database. By applying various stringent criteria, 11 candidate proteins were selected. Among these, we validated an significant increase (p = 0.0018) of melanotransferrin (TRFM) at the plasma level of CRC patients through Western blotting, using 130 plasma samples containing 30 healthy controls, 80 CRC patients, and 20 patients of other diseases. Finally, we measured the expression level of TRFM in 325 plasma samples containing 77 healthy controls and 228 CRC patients (34.6 +/- 4.2 ng/mL and 67.0 +/- 6.4 ng/mL, p < 0.0001) through ELISA and demonstrated the area under the receiver operating characteristic curve of 0.723 (p < 0.0001) with a 92.5% specificity, 48.2% sensitivity, and 95.7% positive predictive value. Furthermore, unlike CEA and PAI-1, up-regulation of TRFM in pathological stages I & II groups compared with stages III & IV groups lead us to expect the use TRFM for early-stage diagnosis of CRC. In this study, we suggest TRFM as a potential serological marker for CRC and expect our discovery strategy to help identify highly cancer-specific and body-fluid-accessible biomarkers. English AMER CHEMICAL SOC PLASMA-PROTEOME CELL-LINES IDENTIFICATION PROTEINS BIOMARKERS COLON EXPRESSION PLASMINOGEN PREDICTION OPPORTUNITIES Discovery of Melanotransferrin as a Serological Marker of Colorectal Cancer by Secretome Analysis and Quantitative Proteomics Article 10.1021/pr500790f 1 JOURNAL OF PROTEOME RESEARCH, v.13, no.11, pp.4919 - 4931 JOURNAL OF PROTEOME RESEARCH 13 11 4919 4931 scie scopus 000344636500039 2-s2.0-84908892144 Biochemical Research Methods Biochemistry & Molecular Biology Article PLASMA-PROTEOME CELL-LINES IDENTIFICATION PROTEINS BIOMARKERS COLON EXPRESSION PLASMINOGEN PREDICTION OPPORTUNITIES melanotransferrin secretome ICAT mTRAQ serological colorectal cancer