Kim, So-Hee Cha, Eun-Ju Lee, Kang Mi Kim, Ho Jun Kwon, Oh-Seung Lee, Jaeick 2024-01-20T08:33:14Z 2024-01-20T08:33:14Z 2021-09-02 2014-11 1942-7603 https://pubs.kist.re.kr/handle/201004/126205 Metal ion coordination ionspray (M+CIS) ionization is a powerful technique to enhance ionization efficiency and sensitivity. In this study, we developed and validated an analytical method for simultaneous ionization and analysis of 84 anabolic androgenic steroids (65 exogenous and 19 endogenous) using liquid chromatography-silver ion coordination ionspray/triple-quadrupole mass spectrometry (LC-Ag+CIS/MS/MS). The concentrations of silver ions and organic solvents have been optimized to increase the amount of silver ion coordinated complexes. A combination of 25M of silver ions and methanol showed the best sensitivity. The validation results showed the intra- (0.8-9.2%) and inter-day (2.5-14.9%) precisions, limits of detection (0.0005-5.0ng/mL), and matrix effect (71.8-100.3%) for the screening analysis. No significant ion suppression was observed. In addition, this method was successfully applied to analysis of positive samples from suspected abusers and useful for the detection of the trace levels of anabolic steroids in human urine samples. Copyright (c) 2014 John Wiley & Sons, Ltd. English John Wiley & Sons Ltd. Simultaneous ionization and analysis of 84 anabolic androgenic steroids in human urine using liquid chromatography-silver ion coordination ionspray/triple-quadrupole mass spectrometry Article 10.1002/dta.1747 1 Drug Testing and Analysis, v.6, no.11-12, pp.1174 - 1185 Drug Testing and Analysis 6 11-12 1174 1185 N scie scopus 000346496000015 2-s2.0-84923207302 Biochemical Research Methods Chemistry, Analytical Pharmacology & Pharmacy Biochemistry & Molecular Biology Chemistry Pharmacology & Pharmacy Article LC-CIS-MS ELECTROSPRAY-IONIZATION SCREENING METHOD PEROXIDATION PRODUCTS POSTCOLUMN REAGENT SPRAY IDENTIFICATION SEPARATION NITRATE DIFFERENTIATION LC-Ag+CIS MS MS anabolic androgenic steroids doping control silver coordination ionization