Jung, Jae Hee Han, Chao Lee, Seung Ah Kim, Jinho Yang, Changhuei 2024-01-20T08:34:08Z 2024-01-20T08:34:08Z 2021-09-02 2014-10-07 1473-0197 https://pubs.kist.re.kr/handle/201004/126249 The fabrication of a novel microfluidic system, integrated with a set of laser-controlled microactuators on an ePetri on-chip microscopy platform, is presented in this paper. In the fully integrated microfluidic system, a set of novel thermally actuated paraffin-based microactuators, precisely controlled by programmed laser optics, was developed to regulate flow and to provide pumping of liquid solutions without external connections. The microfluidic chip was fabricated on a complementary metal-oxide-semiconductor (CMOS)-imaging sensor chip on an ePetri platform; this configuration provided real-time, wide field-of-view, high-resolution imaging using a sub-pixel sweeping microscopy technique. The system of microactuators, which consisted of microvalves and a micropump, operated well in the microfluidic channel with a focused near-infrared laser beam providing the actuation control. As a demonstration, we used our prototype to assess cell-drug interactions and to monitor cell growth directly within an incubator in real time. The powerful combination of laser-actuated microfluidics and chip-scale microscopy techniques represents a significant step forward in terms of a simple, robust, high-throughput, and highly compact analysis system for biomedical and bioscience applications. English Royal Society of Chemistry PHASE-CHANGE MICROVALVES MICROPUMPS DEVICE VALVES FLOW AMPLIFICATION CAMPTOTHECIN POLYMER SYSTEMS Microfluidic-integrated laser-controlled microactuators with on-chip microscopy imaging functionality Article 10.1039/c4lc00790e 1 Lab on a Chip, v.14, no.19, pp.3781 - 3789 Lab on a Chip 14 19 3781 3789 scie scopus 000342650600014 2-s2.0-84906678711 Biochemical Research Methods Chemistry, Multidisciplinary Chemistry, Analytical Nanoscience & Nanotechnology Instruments & Instrumentation Biochemistry & Molecular Biology Chemistry Science & Technology - Other Topics Instruments & Instrumentation Article PHASE-CHANGE MICROVALVES MICROPUMPS DEVICE VALVES FLOW AMPLIFICATION CAMPTOTHECIN POLYMER SYSTEMS Microfluidic Microscopy Laser Microactuator Microvalve Micropump Paraffin Super-resolution On-chip Cell