Kang, Min-Kyoung Eom, Jin-Hee Kim, Yunje Um, Youngsoon Woo, Han Min 2024-01-20T08:34:48Z 2024-01-20T08:34:48Z 2021-09-02 2014-10 0141-5492 https://pubs.kist.re.kr/handle/201004/126284 Pinene is a monoterpenes (C-10) that is produced in a genetically-engineered microbial host for its industrial applications in fragrances, flavoring agents, pharmaceuticals, and biofuels. Herein, we have metabolically-engineered Corynebacterium glutamicum, to produce pinene and studied its toxicity in C. glutamicum. Geranyl diphosphate synthases (GPPS) and pinene synthases (PS), obtained from Pinus taeda and Abies grandis, were co-expressed with over-expressed native 1-deoxy-d-xylulose-5-phosphate synthase (Dxs) and isopentenyl diphosphate isomerase (Idi) from C. glutamicum using CoryneBrick vector. Most strains expressing PS-GPPSs produced detectable amounts of pinene, but co-expression of DXS and IDI with PS (P. taeda) and GPPS (A. grandis) resulted in 27 mu g +/- A 7 alpha-pinene g(-1) cell dry weight, which is the first report in C. glutamicum. Further engineering of PS and GPPS in the C. glutamicum strain may increase pinene production. English Kluwer Academic Publishers Biosynthesis of pinene from glucose using metabolically-engineered Corynebacterium glutamicum Article 10.1007/s10529-014-1578-2 1 Biotechnology Letters, v.36, no.10, pp.2069 - 2077 Biotechnology Letters 36 10 2069 2077 N scie scopus 000341486600019 2-s2.0-84906948633 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article SYNTHETIC BIOLOGY PLATFORM ESCHERICHIA-COLI FUNCTIONAL EXPRESSION CDNA ISOLATION GENE-EXPRESSION ABIES-GRANDIS ALPHA-PINENE OVERPRODUCTION SYNTHASE TOXICITY Corynebacterium Glutamicum Metabolic Engineering Monoterpene Pinene Toxicity