Jang, Yeon Lim Ku, Sook Hee Lee, So Jin Park, Jae Hyung Kim, Won Jong Kwon, Ick Chan Kim, Sun Hwa Jeong, Ji Hoon 2024-01-20T08:34:53Z 2024-01-20T08:34:53Z 2021-09-02 2014-10 1533-4880 https://pubs.kist.re.kr/handle/201004/126289 The clinical applications of therapeutic siRNA remain as a challenge due to the lack of efficient delivery system. In the present study, hyaluronic acid-siRNA conjugate (HA-SS-siRNA)/reducible polyethylenimine (BPEI1.2k-SS) complexes were developed to efficiently deliver the siRNA to HA receptor abundant region with the improved siRNA stability. HA and siRNA were conjugated with disulfide bonds, which are cleavable in cytoplasm. The synthesized HA-SS-siRNA was further complexed with BPEI1.2k-SS, resulting in the formation of spherical nanostructures with approximately 190 nm of size and neutral surface charge. HA-SS-siRNA/BPEI1.2k-SS complexes exhibited the improved stability against serum proteins or polyanions. These complexes were successfully translocated into intracellular region via HA receptor-mediated endocytosis, and silenced target gene expression. English AMER SCIENTIFIC PUBLISHERS LOW-MOLECULAR-WEIGHT INTRACELLULAR DELIVERY GENE DELIVERY DNA DELIVERY CANCER VECTOR CONJUGATE TOXICITY CHITOSAN THERAPY Hyaluronic Acid-siRNA Conjugate/Reducible Polyethylenimine Complexes for Targeted siRNA Delivery Article 10.1166/jnn.2014.9583 1 JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.14, no.10, pp.7388 - 7394 JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 14 10 7388 7394 scie scopus 000336494500004 2-s2.0-84905653657 Chemistry, Multidisciplinary Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Physics, Condensed Matter Chemistry Science & Technology - Other Topics Materials Science Physics Article LOW-MOLECULAR-WEIGHT INTRACELLULAR DELIVERY GENE DELIVERY DNA DELIVERY CANCER VECTOR CONJUGATE TOXICITY CHITOSAN THERAPY siRNA Delivery Hyaluronic Acid Reducible Polyethylenimine Tumor Targeting