Kim, Chloe Galloway, Justin F. Lee, Kwan Hyi Searson, Peter C. 2024-01-20T09:00:32Z 2024-01-20T09:00:32Z 2021-09-02 2014-10 1043-1802 https://pubs.kist.re.kr/handle/201004/126315 Antibodies are a class of molecules widely used in bioengineering and nanomedicine for applications involving protein recognition and targeting. Here we report an efficient method for universal conjugation of antibodies to lipid-coated nanoparticles using radially oriented Fc gamma RIs. This method is performed in physiological solution with no additional coupling reagents, thereby avoiding problems with antibody stability and functionality. Coupling to the Fc region of the antibody avoids aggregation and polymerization allowing high yield. In addition, the antibody is oriented perpendicular to the surface so that the binding sites are fully functional. Using this method we demonstrate quantitative profiling of a panel of four membrane-bound cancer biomarkers (claudin-4, mesothelin, mucin-4, and cadherin-11) on four cell lines (Panc-1, MIA PaCa-2, Capan-1, and HPDE). We show that by designing the lipid coating to minimize aggregation and nonspecific binding, we can obtain absolute values of biomarker expression levels as number per unit area on the cell surface. This method is applicable to a wide range of technologies, including solution based protein detection assays and active targeting of cell surface membrane biomarkers. English AMER CHEMICAL SOC PANCREATIC-CANCER QUANTUM DOTS GENE-EXPRESSION CELL LINES MULTIFUNCTIONAL NANOPARTICLES IGG-BINDING PROTEIN-A ADENOCARCINOMA CLAUDIN-4 MUC4 Universal Antibody Conjugation to Nanoparticles Using the Fc gamma Receptor I (Fc gamma RI): Quantitative Profiling Of Membrane Biomarkers Article 10.1021/bc5003778 1 BIOCONJUGATE CHEMISTRY, v.25, no.10, pp.1893 - 1901 BIOCONJUGATE CHEMISTRY 25 10 1893 1901 scie scopus 000343276400019 2-s2.0-84908028348 Biochemical Research Methods Biochemistry & Molecular Biology Chemistry, Multidisciplinary Chemistry, Organic Biochemistry & Molecular Biology Chemistry Article PANCREATIC-CANCER QUANTUM DOTS GENE-EXPRESSION CELL LINES MULTIFUNCTIONAL NANOPARTICLES IGG-BINDING PROTEIN-A ADENOCARCINOMA CLAUDIN-4 MUC4 FcγReceptor Quantitative Profiling Antibody Conjugation Biomarkers Nanoparticle