Lee, Ji-Hyeok Ko, Ju-Young Oh, Jae-Young Kim, Chul-Young Lee, Hee-Ju Kim, Jaeil Jeon, You-Jin 2024-01-20T09:01:32Z 2024-01-20T09:01:32Z 2021-09-02 2014-09-01 0308-8146 https://pubs.kist.re.kr/handle/201004/126364 d Various bioactive phlorotannins of Ecklonia cave (e.g., dieckol, eckol, 6,6-bieckol, phloroglucinol, phloroeckol, and phlorofucofuroeckol-A) are reported. However, their isolation and purification are not easy. Centrifugal partition chromatography (CPC) can be used to efficiently purify the various bioactive-compounds efficiently from E. cava. Phlorotannins are successfully isolated from the ethyl acetate (EtOAc) fraction of E. cava by CPC with a two-phase solvent system comprising n-hexane:EtOAc:methanol:water (2:7:3:7, v/v) solution. The dieckol (fraction 1, 40.2 mg), phlorofucofuroeckol-A (fraction III, 31.1 mg), and fraction 11 (34.1 mg) with 2,7-phloroglucino1-6,6-bieckol and pyrogallol-phloroglucino1-6,6-bieckol are isolated from the crude extract (500 mg) by a one-step CPC system. The purities of the isolated dieckol and phlorofucofuroeckol-A are >= 90% according to high performance liquid chromatography (HPLC) and electrospray ionization multi stage tandem mass spectrometry analyses. The purified 2,7-phloroglucino1-6,6-bieckol and pyrogallol-phloroglucino1-6,6-bieckol are collected from fraction II by recycle-HPLC. Thus, the CPC system is useful for easy and simple isolation of phlorotannins from E. cava. (C) 2014 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD ANTIOXIDANT PROPERTIES LUNG FIBROBLAST EXTRACT SEPARATION ALGA Preparative isolation and purification of phlorotannins from Ecklonia cava using centrifugal partition chromatography by one-step Article 10.1016/j.foodchem.2014.02.112 1 FOOD CHEMISTRY, v.158, pp.433 - 437 FOOD CHEMISTRY 158 433 437 scie scopus 000335805600061 2-s2.0-84896939612 Chemistry, Applied Food Science & Technology Nutrition & Dietetics Chemistry Food Science & Technology Nutrition & Dietetics Article ANTIOXIDANT PROPERTIES LUNG FIBROBLAST EXTRACT SEPARATION ALGA Centrifugal partition chromatography (CPC) Phlorotannin Ecklonia cava