Lee, Jungwoo Choi, Jong-ryul Ha, Sang Keun Choi, Inwook Lee, Seung Hwan Kim, Donghyun Choi, Nakwon Sung, Jong Hwan 2024-01-20T09:03:14Z 2024-01-20T09:03:14Z 2021-09-02 2014-08-21 1473-0197 https://pubs.kist.re.kr/handle/201004/126452 Various food components are known for their health-promoting effects. However, their biochemical effects are generally evaluated in vitro, and their actual in vivo effect can vary significantly, depending on their metabolic profiles. To evaluate the effect of the liver metabolism on the antioxidant activity, we have developed a two-compartment microfluidic system that integrates the dynamics of liver metabolism and the subsequent antioxidant activity of food components. In the first compartment of the device, human liver enzyme fractions were immobilized inside a poly(ethylene glycol) diacrylate (PEGDA) hydrogel to mimic the liver metabolism. The radical scavenging activity was evaluated by the change of the 2,2-diphenyl-1-picrythydrazyt (DPPH) absorbance in the second compartment. Reaction engineering and fluid mechanics principles were used to develop a simplified analytical model and a more complex finite element model, which were used to design the chip and determine the optimal flow conditions. For real-time measurements of the reaction on a chip, we developed a custom-made photospectrometer system with an LED light source. The developed microfluidic system showed a linear and dose-dependent antioxidant activity in response to increasing concentration of flavonoid. We also compared the antioxidant activity of flavonoid after various liver metabolic reactions. This microfluidic system can serve as a novel in vitro platform for predicting the antioxidant activity of various food components in a more physiologically realistic manner, as well as for studying the mechanism of action of such food components. English Royal Society of Chemistry ON-A-CHIP FLUORESCENCE OPTICAL-DETECTION DRUG-METABOLISM IN-SITU QUERCETIN FLAVONOIDS ENZYMES SYSTEMS MODEL RAT A microfluidic device for evaluating the dynamics of the metabolism-dependent antioxidant activity of nutrients Article 10.1039/c4lc00332b 1 Lab on a Chip, v.14, no.16, pp.2948 - 2957 Lab on a Chip 14 16 2948 2957 scie scopus 000339470400009 2-s2.0-84904355439 Biochemical Research Methods Chemistry, Multidisciplinary Chemistry, Analytical Nanoscience & Nanotechnology Instruments & Instrumentation Biochemistry & Molecular Biology Chemistry Science & Technology - Other Topics Instruments & Instrumentation Article ON-A-CHIP FLUORESCENCE OPTICAL-DETECTION DRUG-METABOLISM IN-SITU QUERCETIN FLAVONOIDS ENZYMES SYSTEMS MODEL RAT