Kim, Su Jin Kim, Eunice EunKyeong 2024-01-20T09:04:44Z 2024-01-20T09:04:44Z 2021-09-05 2014-08 2053-230X https://pubs.kist.re.kr/handle/201004/126531 VCP (valosin-containing protein; also known as p97) plays important roles in many biological processes including the ERAD (endoplasmic reticulum-associated degradation) pathway and its function is governed by binding partners. OTU1 (ovarian tumour domain-containing protein 1) is a recently discovered deubiquitinating enzyme that interacts directly with VCP in the ERAD pathway. In order to understand the interactions between the two proteins, the N-D1 domain of VCP and the UBXL domain of OTU1 were cloned, overexpressed, purified and crystallized. The crystals of the complex diffracted to 3.25 angstrom resolution and belonged to space group P2(1), with unit-cell parameters a = 165.45, b = 176.73, c = 165.59 angstrom, beta = 120.095 degrees. There are two molecules of the complex in the asymmetric unit with a Matthews coefficient of 2.62 angstrom(3) Da(-1) and a solvent content of 53%. English INT UNION CRYSTALLOGRAPHY VALOSIN-CONTAINING PROTEIN ENDOPLASMIC-RETICULUM BINDING P97 REVEALS SYSTEM MODE Crystallization and preliminary X-ray crystallographic analysis of the complex between the N-D1 domain of VCP from Homo sapiens and the N domain of OTU1 from Saccharomyces cerevisiae Article 10.1107/S2053230X14013351 1 ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, v.70, pp.1087 - 1089 ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 70 1087 1089 scopus 000340246500018 2-s2.0-84905497379 Biochemical Research Methods Biochemistry & Molecular Biology Biophysics Crystallography Biochemistry & Molecular Biology Biophysics Crystallography Article VALOSIN-CONTAINING PROTEIN ENDOPLASMIC-RETICULUM BINDING P97 REVEALS SYSTEM MODE ATPase OTU1 UBXL domain VCP