Kang, Suk Woo Kang, Kyungsu Kim, Mi Ae Jeon, Na Ra Kim, Sang Min Jeon, Je-Seung Nho, Chu Won Um, Byung-Hun 2024-01-20T09:30:24Z 2024-01-20T09:30:24Z 2021-09-04 2014-08 1438-2377 https://pubs.kist.re.kr/handle/201004/126561 Aceriphyllum rossii Engler (Saxifragaceae) has been used as a nutritious food in Korea, but only minimal studies on this plant have been undertaken. We have examined the estrogenic effect of A. rossii and have discovered its potent constituents. To identify the estrogenic activity of A. rossii, we measured the transactivation of the estrogen-responsive element (ERE) in MCF-7 cells transfected with an ERE-containing construct after treatment of the extract and fractions of A. rossii. A. rossii showed a potent estrogenic activity and gallic acid ethyl ester; quercetin and kaempferol were identified as major constituents of the active fractions by liquid chromatography-nuclear magnetic resonance spectroscopy/mass spectrometry. We confirmed quercetin and kaempferol are the major active constituents after activity-guided isolation of seven compounds: gallic acid ethyl ester (1), quercetin (2), kaempferol (3), quercetin-3-O-(6aEuro(3)-galloyl)-beta-d-glucopyranoside (4), quercetin-3-O-beta-d-glucopyranoside (5), kaempferol-3-O-(6aEuro(3)-galloyl)-beta-d-glucopyranoside (6), and kaempferol-3-O-beta-d-glucopyranoside (7). These results show A. rossii could be a possible candidate for the treatment of postmenopausal symptoms. English SPRINGER MAGNETIC-RESONANCE-SPECTROSCOPY BETA-GLUCOSIDASE ACTIVITY ESTROGEN-RECEPTOR-ALPHA LIQUID-CHROMATOGRAPHY ANTIOXIDANT ACTIVITY FLAVONOL GLYCOSIDES PHENOLIC-COMPOUNDS SMALL-INTESTINE QUERCETIN PLANT Phytoestrogenic activity of Aceriphyllum rossii and rapid identification of phytoestrogens by LC-NMR/MS and bioassay-guided isolation Article 10.1007/s00217-014-2212-1 1 EUROPEAN FOOD RESEARCH AND TECHNOLOGY, v.239, no.2, pp.237 - 246 EUROPEAN FOOD RESEARCH AND TECHNOLOGY 239 2 237 246 scie scopus 000339339200007 2-s2.0-84904392250 Food Science & Technology Food Science & Technology Article MAGNETIC-RESONANCE-SPECTROSCOPY BETA-GLUCOSIDASE ACTIVITY ESTROGEN-RECEPTOR-ALPHA LIQUID-CHROMATOGRAPHY ANTIOXIDANT ACTIVITY FLAVONOL GLYCOSIDES PHENOLIC-COMPOUNDS SMALL-INTESTINE QUERCETIN PLANT Aceriphyllum rossii Phytoestrogenic activity LC-NMR/MS Quercetin Kaempferol