Kang, Min-Kyoung Lee, Jungseok Um, Youngsoon Lee, Taek Soon Bott, Michael Park, Si Jae Woo, Han Min 2024-01-20T09:31:36Z 2024-01-20T09:31:36Z 2021-09-05 2014-07 0175-7598 https://pubs.kist.re.kr/handle/201004/126618 Currently, the majority of tools in synthetic biology have been designed and constructed for model organisms such as Escherichia coli and Saccharomyces cerevisiae. In order to broaden the spectrum of organisms accessible to such tools, we established a synthetic biological platform, called CoryneBrick, for gene expression in Corynebacterium glutamicum as a set of E. coli-C. glutamicum shuttle vectors whose elements are interchangeable with BglBrick standard parts. C. glutamicum is an established industrial microorganism for the production of amino acids, proteins, and commercially promising chemicals. Using the CoryneBrick vectors, we showed various time-dependent expression profiles of a red fluorescent protein. This CoryneBrick platform was also applicable for two-plasmid expression systems with a conventional C. glutamicum expression vector. In order to demonstrate the practical application of the CoryneBrick vectors, we successfully reconstructed the xylose utilization pathway in the xylose-negative C. glutamicum wild type by fast BglBrick cloning methods using multiple genes encoding for xylose isomerase and xylulose kinase, resulting in a growth rate of 0.11 +/- 0.004 h(-1) and a xylose uptake rate of 3.35 mmol/gDW/h when 1 % xylose was used as sole carbon source. Thus, CoryneBrick vectors were shown to be useful engineering tools in order to exploit Corynebacterium as a synthetic platform for the production of chemicals by controllable expression of the genes of interest. English Springer Verlag Synthetic biology platform of CoryneBrick vectors for gene expression in Corynebacterium glutamicum and its application to xylose utilization Article 10.1007/s00253-014-5714-7 1 Applied Microbiology and Biotechnology, v.98, no.13, pp.5991 - 6002 Applied Microbiology and Biotechnology 98 13 5991 6002 N scie scopus 000337747900018 2-s2.0-84903560303 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article ESCHERICHIA-COLI ORGANIC-ACIDS PRECURSOR PATHWAY DNA OVERPRODUCTION OPTIMIZATION PROMOTERS STRATEGY PRODUCT Corynebacterium glutamicum Synthetic biology Metabolic engineering BglBrick