Jeong, Yun-Jeong Choi, Yongsoo Shin, Jae-Moon Cho, Hyun-Ji Kang, Jeong-Han Park, Kwan-Kyu Choe, Jung-Yoon Bae, Young-Seuk Han, Sang-Mi Kim, Cheorl-Ho Chang, Hyeun-Wook Chang, Young-Chae 2024-01-20T09:33:49Z 2024-01-20T09:33:49Z 2021-09-05 2014-06 0278-6915 https://pubs.kist.re.kr/handle/201004/126731 Bee venom is a natural compound produced by the honey bee (Apis mellifera), and has been reported as having the biological and pharmacological activities, including anti-bacterial, anti-viral and anti-inflammation. In the present study, the inhibitory effects of bee venom and its major peptide components on the tumor invasion were demonstrated. It was confirmed the inhibitory effects of bee venom, melittin, and apamin on the EGF-induced invasion of breast cancer cells. Transwell invasion and wound-healing assays showed that bee venom and melittin significantly inhibits the EGF-induced invasion and migration of breast cancer cells. Also, bee venom and melittin reduced the EGF-stimulated F-actin reorganization at the leading edge, but apamin did not affect. Particularly, melittin inhibited the EGF-induced MMP-9 expression via blocking the NF-kappa B and PI3K/Akt/mTOR pathway. In addition, melittin significantly suppressed the EGF-induced FAR phosphorylation through inhibition of mTOR/p70S6K/4E-BP1 pathway. These results suggest that inhibitory effects of melittin on breast cancer cell motility and migration may be related to the inhibition of mTOR pathway. (C) 2014 Elsevier Ltd. All rights reserved. English PERGAMON-ELSEVIER SCIENCE LTD FOCAL-ADHESION KINASE NF-KAPPA-B BEE VENOM MMP-9 EXPRESSION CARCINOMA-CELLS OVARIAN-CANCER MIGRATION ACTIVATION INDUCTION RECEPTOR Melittin suppresses EGF-induced cell motility and invasion by inhibiting PI3K/Akt/mTOR signaling pathway in breast cancer cells Article 10.1016/j.fct.2014.03.022 1 FOOD AND CHEMICAL TOXICOLOGY, v.68, pp.218 - 225 FOOD AND CHEMICAL TOXICOLOGY 68 218 225 scie scopus 000337653000023 2-s2.0-84919871325 Food Science & Technology Toxicology Food Science & Technology Toxicology Article FOCAL-ADHESION KINASE NF-KAPPA-B BEE VENOM MMP-9 EXPRESSION CARCINOMA-CELLS OVARIAN-CANCER MIGRATION ACTIVATION INDUCTION RECEPTOR Bee venom Melittin FAK MMP Cancer invasion