Chang, Ki-Young Woo, Doyeon Jung, Hyunjin Lee, Sangkyu Kim, Sungsoo Won, Joungha Kyung, Taeyoon Park, Hyerim Kim, Nury Yang, Hee Won Park, Jae-Yong Hwang, Eun Mi Kim, Daesoo Do Heo, Won 2024-01-20T09:33:55Z 2024-01-20T09:33:55Z 2021-09-05 2014-06 2041-1723 https://pubs.kist.re.kr/handle/201004/126736 Receptor tyrosine kinases (RTKs) are a family of cell-surface receptors that have a key role in regulating critical cellular processes. Here, to understand and precisely control RTK signalling, we report the development of a genetically encoded, photoactivatable Trk (tropomyosin-related kinase) family of RTKs using a light-responsive module based on Arabidopsis thaliana cryptochrome 2. Blue-light stimulation (488 nm) of mammalian cells harbouring these receptors robustly upregulates canonical Trk signalling. A single light stimulus triggers transient signalling activation, which is reversibly tuned by repetitive delivery of blue-light pulses. In addition, the light-provoked process is induced in a spatially restricted and cell-specific manner. A prolonged patterned illumination causes sustained activation of extracellular signal-regulated kinase and promotes neurite outgrowth in a neuronal cell line, and induces filopodia formation in rat hippocampal neurons. These light-controllable receptors are expected to create experimental opportunities to spatiotemporally manipulate many biological processes both in vitro and in vivo. English NATURE PUBLISHING GROUP NERVE GROWTH-FACTOR PROTEIN INTERACTIONS TRK RECEPTORS ACTIVATION INDUCTION CELLS TRANSDUCTION DIFFERENTIATION PARTNERS LIGAND Light-inducible receptor tyrosine kinases that regulate neurotrophin signalling Article 10.1038/ncomms5057 1 NATURE COMMUNICATIONS, v.5 NATURE COMMUNICATIONS 5 scie scopus 000338838000001 2-s2.0-84901992638 Multidisciplinary Sciences Science & Technology - Other Topics Article NERVE GROWTH-FACTOR PROTEIN INTERACTIONS TRK RECEPTORS ACTIVATION INDUCTION CELLS TRANSDUCTION DIFFERENTIATION PARTNERS LIGAND Biological sciences Bioengineering Cell biology