Lee, C. Justin Min, Joo Ok Yoon, Bo-Eun 2024-01-20T09:34:00Z 2024-01-20T09:34:00Z 2021-09-05 2014-06 1976-8354 https://pubs.kist.re.kr/handle/201004/126741 We demonstrate that pronuclear injection of lentiviral vectors expressing small hairpin RNAs (shRNAs) that silence the expression of specific genes can be used to generate knockdown mice. A lentiviral vector capable of generating shRNA that is specific for the target gene bestrophin 1 (Best1) encodes for an anion channel that is permeable to glutamate and gamma amino butyric acid (GABA) and that also regulates intracellular calcium signaling. We confirmed that cultured cerebellar glia from these Best1 knockdown mice showed attenuation of GABA release induced by an increase in intracellular calcium. Therefore, we propose that a combined approach, the use of transgenesis together with lentiviral vectors expressing shRNAs, can successfully generate a large number of mice in which the expression of a specific gene can be downregulated gradually. We also suggest that the Best1 knockdown mouse can be a useful tool for studying Best1 gene function. English TAYLOR & FRANCIS LTD INHIBITION CHANNEL Generation of Best1 knockdown mice using lentiviral vector expressing small hairpin RNA Article 10.1080/19768354.2014.924994 1 ANIMAL CELLS AND SYSTEMS, v.18, no.3, pp.178 - 182 ANIMAL CELLS AND SYSTEMS 18 3 178 182 scie scopus kci 000337965500003 2-s2.0-84902813632 Cell Biology Zoology Cell Biology Zoology Article INHIBITION CHANNEL astrocyte cerebellum tonic inhibition