Lee, Sangmin Koo, Heebeom Na, Jin Hee Lee, Kyung Eun Jeong, Seo Young Choi, Kuiwon Kim, Sun Hwa Kwon, Ick Chan Kim, Kwangmeyung 2024-01-20T10:00:30Z 2024-01-20T10:00:30Z 2021-09-05 2014-05 1936-0851 https://pubs.kist.re.kr/handle/201004/126814 In general, traditional gene carriers contain strong cationic charges to efficiently load anionic genes, but this cationic character also leads to destabilization of plasma membranes and causes severe cytotoxicity. Here, we developed a PCR-based nanofactory as a safe gene delivery system. A few template plasmid DNA can be amplified by PCR inside liposomes about 200 nm in diameter, and the quantity of loaded genes highly Increased by more than 8.8-fold. The liposome membrane was composed of neutral lipids free from cationic charges. Consequently, this system is nontoxic, unlike other traditional cationic gene carriers. Intense red fluorescent protein (REP) expression in CHO-K1 cells showed that the amplified genes could be successfully transfected to cells. Animal experiments with the luciferase gene also showed in vivo gene expression by our system without toxicity. We think that this PCR-based nanofactory system can overcome the toxicity problem that is the critical limitation of current gene delivery to clinical application. English AMER CHEMICAL SOC TARGETED DELIVERY IN-VIVO PROTEIN NANOPARTICLES THERAPY DNA Amplification in Neutral Liposomes for Safe and Efficient Gene Delivery Article 10.1021/nn501106a 1 ACS NANO, v.8, no.5, pp.4257 - 4267 ACS NANO 8 5 4257 4267 scie scopus 000336640600017 2-s2.0-84901659599 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Chemistry Science & Technology - Other Topics Materials Science Article TARGETED DELIVERY IN-VIVO PROTEIN NANOPARTICLES THERAPY polymerase chain reaction neutral liposomes gene delivery nanofactory