Yun, Yeo-Myeong Kim, Dong-Hoon Oh, You-Kwan Shin, Hang-Sik Jung, Kyung-Won 2024-01-20T10:01:04Z 2024-01-20T10:01:04Z 2021-09-05 2014-05 0960-8524 https://pubs.kist.re.kr/handle/201004/126843 In this study, a novel enzymatic pretreatment of Chlorella vulgaris for dark fermentative hydrogen production (DFHP) was performed using crude hydrolytic extracellular enzyme solution (CHEES) extracted from the H-2 fermented effluent of food waste. It was found that the enzyme extracted at 52 h had the highest hydrolysis efficiency of microalgal biomass, resulting in the highest H-2 yield of 43.1 mL H-2/g dry cell weight along with shorter lag periods. Even though a high amount of VFAs was accumulated in CHEES, especially butyrate, the fermentative bacteria on the DFHP was not affected from product inhibition. It also appears that the presence of organic acids, especially lactate and acetate, contained in the CHEES facilitated enhancement of H-2 production acted as a co-substrate. Therefore, all of the experimental results suggest that the enhancement of DFHP performance caused by CHEES has a dual role as the hydrolysis enhancer and the co-substrate supplier. (C) 2014 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD WASTE-WATER ENHANCEMENT SUBSTRATE Application of a novel enzymatic pretreatment using crude hydrolytic extracellular enzyme solution to microalgal biomass for dark fermentative hydrogen production Article 10.1016/j.biortech.2014.02.129 1 BIORESOURCE TECHNOLOGY, v.159, pp.365 - 372 BIORESOURCE TECHNOLOGY 159 365 372 scie scopus 000335393500048 2-s2.0-84897847779 Agricultural Engineering Biotechnology & Applied Microbiology Energy & Fuels Agriculture Biotechnology & Applied Microbiology Energy & Fuels Article WASTE-WATER ENHANCEMENT SUBSTRATE Chlorella vulgaris Food waste Crude hydrolytic extracellular enzyme solution Co-substrate